ArticleGut2002
Stimulation of the intestinal Cdx2 homeobox gene by butyrate in colon cancer cells.
Article in Gut, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 65 citations in OpenAlex.
- Effect of Tributyrin on Growth Performance and Pathway by which Tributyrin Regulates Oligopeptide Transporter 1 in Juvenile Grass Carp (Animals : an open access journal from MDPI · 2022Article
- Transcriptional programmes underlying cellular identity and microbial responsiveness in the intestinal epithelium.Nature reviews. Gastroenterology & hepatology · 2021Review
- Ketogenesis contributes to intestinal cell differentiation.Cell death and differentiation · 2017Article
- Intestinal microbiota: a potential target for the treatment of postmenopausal osteoporosis.Bone research · 2017Review
- Immature myeloid progenitors promote disease progression in a mouse model of Barrett's-like metaplasia.Oncotarget · 2015Article
- Achaete scute-like 2 suppresses CDX2 expression and inhibits intestinal neoplastic epithelial cell differentiation.Oncotarget · 2015Article
- IGFBP-rP1, a potential molecule associated with colon cancer differentiation.Molecular cancer · 2010Article
- Inhibition of nucleostemin upregulates CDX2 expression in HT29 cells in response to bile acid exposure: implications in the pathogenesis of Barrett's esophagus.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2009Article
- Butyrate transcriptionally enhances peptide transporter PepT1 expression and activity.PloS one · 2008Article
- The epigenetic signature of CFTR expression is co-ordinated via chromatin acetylation through a complex intronic element.The Biochemical journal · 2007Article
- Intestinal apoptotic changes linked to metabolic status in fasted and refed rats.Pflugers Archiv : European journal of physiology · 2006Article
- Article
- Phosphorylation of the homeotic tumor suppressor Cdx2 mediates its ubiquitin-dependent proteasome degradation.Oncogene · 2005Article
- Immunochemical, biomolecular and biochemical characterization of bovine epithelial intestinal primocultures.BMC cell biology · 2005Article
- The homeodomain protein CDX2 is an early marker of Barrett's oesophagus.Journal of clinical pathology · 2004Article
- Cdx2 is essential for axial elongation in mouse development.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Homeobox genes: going for growth.Gut · 2002Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe transcription factor encoded by the intestinal Cdx2 homeobox gene and treatment with sodium butyrate (NaB), a byproduct of fibre fermentation by colonic bacteria, exert similar effects on colon cancer cell lines as they both inhibit cell growth and stimulate cell differentiation and apoptosis.
aimTo investigate whether NaB regulates expression of the Cdx2 gene in colon cancer cell lines.
methodsHuman adenocarcinoma cell lines Caco2 and HT29 were grown in the presence or absence of NaB. Cells were analysed for Cdx2 mRNA expression by reverse transcription-polymerase chain reaction, for protein expression by western blotting and electromobility shift assays, and for transcriptional activity of the Cdx2 promoter by transfection with luciferase reporter plasmids.
resultsIn HT29 and Caco2 cells, NaB stimulated Cdx2 mRNA and protein expression as well as transcriptional activity of the Cdx2 promoter. Stimulation of the activity of the Cdx2 promoter by NaB was dose and time dependent. The Cdx2 promoter contains discrete regions that participate in or inversely that blunt the stimulatory effect exerted by NaB. In addition, NaB stimulated the transcriptional activity of the Cdx2 promoter downregulated by oncogenic ras.
conclusionThis study is the first report of an intestine specific transcription factor, Cdx2, stimulated by butyrate. Thus it provides a new mechanism whereby butyrate controls proliferation and differentiation of colon cancer cells.
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