ArticleThe EMBO journal2002
Cell cycle-dependent regulation of the association between origin recognition proteins and somatic cell chromatin.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 46 citations in OpenAlex.
- Starting DNA Synthesis: Initiation Processes during the Replication of Chromosomal DNA in Humans.Genes · 2024Review
- A new class of disordered elements controls DNA replication through initiator self-assembly.eLife · 2019Article
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- Orc1 Binding to Mitotic Chromosomes Precedes Spatial Patterning during G1 Phase and Assembly of the Origin Recognition Complex in Human Cells.The Journal of biological chemistry · 2015Article
- Regulating DNA replication in eukarya.Cold Spring Harbor perspectives in biology · 2013Review
- Orc2 protects ORCA from ubiquitin-mediated degradation.Cell cycle (Georgetown, Tex.) · 2012Article
- Phosphorylation of ORC2 protein dissociates origin recognition complex from chromatin and replication origins.The Journal of biological chemistry · 2012Article
- "The Octet": Eight Protein Kinases that Control Mammalian DNA Replication.Frontiers in physiology · 2012Article
- Interactions of a replication initiator with histone H1-like proteins remodel the condensed mitochondrial genome.The Journal of biological chemistry · 2011Article
- Mammalian sperm chromatin as a model for chromatin function in DNA degradation and DNA replication.Systems biology in reproductive medicine · 2011Review
- A WD-repeat protein stabilizes ORC binding to chromatin.Molecular cell · 2010Article
- Article
- Butyrate induced cell cycle arrest in bovine cells through targeting gene expression relevant to DNA replication apparatus.Gene regulation and systems biology · 2008Article
- Article
- Cell cycle regulation of DNA replication.Annual review of genetics · 2007Review
- Butyrate induces profound changes in gene expression related to multiple signal pathways in bovine kidney epithelial cells.BMC genomics · 2006Article
- DNA replication and progression through S phase.Oncogene · 2005Review
- Human Orc2 localizes to centrosomes, centromeres and heterochromatin during chromosome inheritance.The EMBO journal · 2004Article
- Role for Cdk1 (Cdc2)/cyclin A in preventing the mammalian origin recognition complex's largest subunit (Orc1) from binding to chromatin during mitosis.Molecular and cellular biology · 2004Article
- The role of Cdc6 in ensuring complete genome licensing and S phase checkpoint activation.The Journal of cell biology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Previous studies have suggested that cell cycle-dependent changes in the affinity of the origin recognition complex (ORC) for chromatin are involved in regulating initiation of DNA replication. To test this hypothesis, chromatin lacking functional ORCs was isolated from metaphase hamster cells and incubated in Xenopus egg extracts to initiate DNA replication. Intriguingly, Xenopus ORC rapidly bound to hamster somatic chromatin in a Cdc6-dependent manner and was then released, concomitant with initiation of DNA replication. Once pre-replication complexes (pre-RCs) were assembled either in vitro or in vivo, further binding of XlORC was inhibited. Neither binding nor release of XlORC was affected by inhibitors of either cyclin-dependent protein kinase activity or DNA synthesis. In contrast, inhibition of pre-RC assembly, either by addition of Xenopus geminin or by depletion of XlMcm proteins, augmented ORC binding by inhibiting ORC release. These results demonstrate a programmed release of XlORC from somatic cell chromatin as it enters S phase, consistent with the proposed role for ORC in preventing re-initiation of DNA replication during S phase.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.