ArticleJournal of biomolecular NMR2002
MQ-hCN-based pulse sequences for the measurement of 13C1'-1H1', 13C1'-15N, 1H1'-15N, 13C1'-13C2', 1H1'-13C2',13C6/8-1H6/8, 13C6/8-15N, 1H6/8-15N, 13C6-13C5, 1H6-13C5 dipolar couplings in 13C, 15N-labeled DNA (and RNA).
Article in Journal of biomolecular NMR, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 20 citations in OpenAlex.
- Advances that facilitate the study of large RNA structure and dynamics by nuclear magnetic resonance spectroscopy.Wiley interdisciplinary reviews. RNA · 2019Review
- Sugar-to-base correlation in nucleic acids with a 5D APSY-HCNCH or two 3D APSY-HCN experiments.Journal of biomolecular NMR · 2012Article
- Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings.Nature protocols · 2007Article
- Resolution-enhanced base-type-edited HCN experiment for RNA.Journal of biomolecular NMR · 2005Article
- Accurate measurement of 15N-13C residual dipolar couplings in nucleic acids.Journal of biomolecular NMR · 2005Article
- Resolution-optimized NMR measurement of (1)D(CH), (1)D(CC) and (2)D(CH) residual dipolar couplings in nucleic acid bases.Journal of biomolecular NMR · 2004Article
- Experiments for correlating quaternary carbons in RNA bases.Journal of biomolecular NMR · 2004Article
- New applications of 2D filtered/edited NOESY for assignment and structure elucidation of RNA and RNA-protein complexes.Journal of biomolecular NMR · 2004Article
- Direct observation of dipolar couplings between distant protons in weakly aligned nucleic acids.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Measurement of H2'-C2' and H3'-C3' dipolar couplings in RNA molecules.Journal of biomolecular NMR · 2002Article
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4 authors at 1 institution in 1 country.
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Abstract
A suite of multiple quantum (MQ) HCN-based pulse sequences has been developed for the purpose of collecting dipolar coupling data in labeled nucleic acids. All the pulse sequences are based on the robust MQ-HCN experiment which has been utilized for assignment purposes in labeled nucleic acids for a number of years and provides much-needed resolution for the dipolar coupling measurements. We have attempted to collect multiple couplings centered on the 13C1' and 13C6/8 positions. Six pulse sequences are described, one each for measurement of one-bond 13C1'-1H1' and 13C6/8-1H6/8 couplings, one for measurement of one-bond 13C1'-15N and two-bond 1H1'-15N couplings, one for measurement of one-bond 13C6/8-15N and two-bond 1H6/8-15N couplings, one for measurement of one-bond 13C1'- 13C2' and two-bond 1H1'-13C2' couplings, and one for measurement of one-bond 13C6-13C5 and two-bond 1H6-13C5 couplings in the bases of C and T. These sequences are demonstrated for a labeled 18 bp DNA duplex in a 47 kDa ternary complex of DNA, CBFbeta, and the CBFalpha Runt domain, thus clearly demonstrating the robustness of the pulse sequences even for a very large complex.
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