ArticleNucleic acids research2002
A rapid, quantitative, non-radioactive bisulfite-SNuPE- IP RP HPLC assay for methylation analysis at specific CpG sites.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Clinicopathological Significance of CDKN2A Promoter Hypermethylation Frequency with Pancreatic Cancer.Scientific reports · 2015Pooled it
- The common bisulfite-conversion-based techniques to analyze DNA methylation in human cancers.Cancer cell international · 2024Review
- Bisulfite profiling of the MGMT promoter and comparison with routine testing in glioblastoma diagnostics.Clinical epigenetics · 2022Article
- DNA Methylation in Solid Tumors: Functions and Methods of Detection.International journal of molecular sciences · 2021Review
- Detailed methylation map of LINE-1 5'-promoter region reveals hypomethylated CpG hotspots associated with tumor tissue specificity.Molecular genetics & genomic medicine · 2019Article
- Inter-locus as well as intra-locus heterogeneity in LINE-1 promoter methylation in common human cancers suggests selective demethylation pressure at specific CpGs.Clinical epigenetics · 2015Article
- 11p15 DNA-methylation analysis in monozygotic twins with discordant intrauterine development due to severe twin-to-twin transfusion syndrome.Clinical epigenetics · 2014Article
- Adult monozygotic twins discordant for intra-uterine growth have indistinguishable genome-wide DNA methylation profiles.Genome biology · 2013Article
- Strategies for discovery and validation of methylated and hydroxymethylated DNA biomarkers.Cancer medicine · 2012Review
- Methylation of L1Hs promoters is lower on the inactive X, has a tendency of being higher on autosomes in smaller genomes and shows inter-individual variability at some loci.Human molecular genetics · 2012Article
- Whole blood DNA aberrant methylation in pancreatic adenocarcinoma shows association with the course of the disease: a pilot study.PloS one · 2012Article
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- Association of COMT genotypes with S-COMT promoter methylation in growth-discordant monozygotic twins and healthy adults.BMC medical genetics · 2011Article
- MIRA-SNuPE, a quantitative, multiplex method for measuring allele-specific DNA methylation.Epigenetics · 2011Article
- Methylation at global LINE-1 repeats in human blood are affected by gender but not by age or natural hormone cycles.PloS one · 2011Article
- Fluorescent conjugated polymer-based FRET technique for detection of DNA methylation of cancer cells.Nature protocols · 2010Article
- Specificity of methylation assays in cancer research: a guideline for designing primers and probes.Obstetrics and gynecology international · 2010Article
- CpG methylation analysis--current status of clinical assays and potential applications in molecular diagnostics: a report of the Association for Molecular Pathology.The Journal of molecular diagnostics : JMD · 2009Article
- MethMarker: user-friendly design and optimization of gene-specific DNA methylation assays.Genome biology · 2009Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The precise mapping and quantification of DNA methylation as an epigenetic parameter during development and in diseased tissues is of great importance for functional genomics. Here we describe a rapid, quantitative method to assess methylation levels at specific CpG sites using PCR products of bisulfite-treated genomic DNA. Using single nucleotide primer extension (SNuPE) assays in combination with ion pair reverse phase high performance liquid chromatography (IP RP HPLC) separation techniques, methylated and unmethylated CpGs can be discriminated and quantified based on the different masses and hydrophobicities of the extended primer products. The assay is linear, highly reproducible and several sites can be measured simultaneously in one reaction. It can be semi-automated and eliminates the need for cloning and sequencing of individual bisulfite PCR products.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.