ReviewNucleic acids research2002
Real-time PCR in virology.
Review in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 390 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
390 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Serum and urine nucleic acid screening tests for BK polyomavirus-associated nephropathy in kidney and kidney-pancreas transplant recipients.The Cochrane database of systematic reviews · 2024Pooled it
- Prevalence of human T-lymphotropic virus type 1 and 2 (HTLV-1/-2) infection in pregnant women in Brazil: a systematic review and meta-analysis.Scientific reports · 2021Pooled it
- Evaluation of Universal Biomolecular Medium for Molecular Detection of Respiratory Viruses: A Paired, Multi-Platform Comparison.Microorganisms · 2026Article
- Molecular Diagnostics of Infectious Diseases.Yonago acta medica · 2026Review
- Detection Strategies for Begomoviruses: A Focus Review of Methods.Molecular biotechnology · 2026Review
- An Optimized RT-qPCR Protocol for Comprehensive Analysis of microRNAs and mRNAs inNon-coding RNA · 2026Article
- Accurate and sensitive TaqMan real-time PCR assays to detect and quantify simian malaria parasites Plasmodium cynomolgi, Plasmodium inui and Plasmodium coatneyi.Malaria journal · 2026Article
- Development of a Multiplex TaqMan Real-Time PCR Assay for the Simultaneous Detection and Differentiation of Three PathogenicVeterinary sciences · 2026Article
- Article
- Rapid and simultaneous detection ofMicrobiology spectrum · 2026Article
- Shedding light on deception: LAMP as a tool for detecting adulterants in herbal medicine.Protoplasma · 2026Review
- Evaluation of diagnostic performance of comprehensive respiratory virus panel: comparison of next-generation-sequencing to real-time polymerase chain reaction for detection of respiratory viruses.Annals of clinical microbiology and antimicrobials · 2026Article
- Long-Term Stable Biosensing Using Multiscale Biostructure-Preserving Metal Thin Films.Biosensors · 2026Article
- Validation of a multiplex qRT-PCR assay for the detection of RSV, influenza A/B virus and SARS-CoV-2.BMC microbiology · 2026Article
- Serum as an alternative matrix for reliable molecular diagnosis of fowl adenovirus infections.Frontiers in veterinary science · 2026Article
- Rapid detection of human and animal respiratory viruses using Microbe Finder (MiFiFrontiers in microbiology · 2026Article
- A duplex real-time fluorescent quantitative PCR method for simultaneous and rapid detection ofFrontiers in veterinary science · 2026Article
- Reactive Quantum Dot-Based FRET Systems for Target-Catalyzed Detection of RNA.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Development and clinical application of a TaqMan-based real-time quantitative PCR assay for rapid detection of goose adenovirus type 4.Frontiers in microbiology · 2026Article
- Article
330 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of the polymerase chain reaction (PCR) in molecular diagnostics has increased to the point where it is now accepted as the gold standard for detecting nucleic acids from a number of origins and it has become an essential tool in the research laboratory. Real-time PCR has engendered wider acceptance of the PCR due to its improved rapidity, sensitivity, reproducibility and the reduced risk of carry-over contamination. There are currently five main chemistries used for the detection of PCR product during real-time PCR. These are the DNA binding fluorophores, the 5' endonuclease, adjacent linear and hairpin oligoprobes and the self-fluorescing amplicons, which are described in detail. We also discuss factors that have restricted the development of multiplex real-time PCR as well as the role of real-time PCR in quantitating nucleic acids. Both amplification hardware and the fluorogenic detection chemistries have evolved rapidly as the understanding of real-time PCR has developed and this review aims to update the scientist on the current state of the art. We describe the background, advantages and limitations of real-time PCR and we review the literature as it applies to virus detection in the routine and research laboratory in order to focus on one of the many areas in which the application of real-time PCR has provided significant methodological benefits and improved patient outcomes. However, the technology discussed has been applied to other areas of microbiology as well as studies of gene expression and genetic disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.