ArticleMolecular and cellular biology2002
CENP-A, -B, and -C chromatin complex that contains the I-type alpha-satellite array constitutes the prekinetochore in HeLa cells.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.
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Who cites it
94 citing papers in PubMed, 172 citations in OpenAlex.
- Review
- KIF18A promotes chromosome congression in cooperation with CENP-E downstream of CENP-C.Cell reports · 2025Article
- Genomic and epigenomic maps of mouse centromeres and pericentromeres.Nature communications · 2025Article
- Conservation of dichromatin organization along regional centromeres.Cell genomics · 2025Article
- CENP-C-Mis12 complex establishes a regulatory loop through Aurora B for chromosome segregation.Life science alliance · 2025Article
- Genes and genetics belong to maize haploid induction.Frontiers in plant science · 2025Review
- Analysis of CENP-B Boxes as Anchor of Kinetochores in Centromeres of Human Chromosomes.Bioinformatics and biology insights · 2024Article
- Satellite RNAs: emerging players in subnuclear architecture and gene regulation.The EMBO journal · 2023Review
- Structure of the human inner kinetochore CCAN complex and its significance for human centromere organization.Molecular cell · 2022Article
- Molecular Complexes at Euchromatin, Heterochromatin and Centromeric Chromatin.International journal of molecular sciences · 2021Review
- Assembly principles and stoichiometry of a complete human kinetochore module.Science advances · 2021Article
- Sequence, Chromatin and Evolution of Satellite DNA.International journal of molecular sciences · 2021Review
- Induction of spontaneous human neocentromere formation and long-term maturation.The Journal of cell biology · 2021Article
- Drosophila Satellite Repeats at the Intersection of Chromatin, Gene Regulation and Evolution.Progress in molecular and subcellular biology · 2021Article
- H3K9me3 maintenance on a human artificial chromosome is required for segregation but not centromere epigenetic memory.Journal of cell science · 2020Article
- Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin.Nature communications · 2020Article
- CDK1-mediated CENP-C phosphorylation modulates CENP-A binding and mitotic kinetochore localization.The Journal of cell biology · 2019Article
- Discovery of 33mer in chromosome 21 - the largest alpha satellite higher order repeat unit among all human somatic chromosomes.Scientific reports · 2019Article
- In vitro BioID: mapping the CENP-A microenvironment with high temporal and spatial resolution.Molecular biology of the cell · 2019Article
- High-resolution mapping of centromeric protein association using APEX-chromatin fibers.Epigenetics & chromatin · 2018Article
34 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CENP-A is a component of centromeric chromatin and defines active centromere regions by forming centromere-specific nucleosomes. We have isolated centromeric chromatin containing the CENP-A nucleosome, CENP-B, and CENP-C from HeLa cells using anti-CENP-A and/or anti-CENP-C antibodies and shown that the CENP-A/B/C complex is predominantly formed on alpha-satellite DNA that contains the CENP-B box (alphaI-type array). Mapping of hypersensitive sites for micrococcal nuclease (MNase) digestion indicated that CENP-A nucleosomes were phased on the alphaI-type array as a result of interactions between CENP-B and CENP-B boxes, implying a repetitive configuration for the CENP-B/CENP-A nucleosome complex. Molecular mass analysis by glycerol gradient sedimentation showed that MNase digestion released a CENP-A/B/C chromatin complex of three to four nucleosomes into the soluble fraction, suggesting that CENP-C is a component of the repetitive CENP-B/CENP-A nucleosome complex. Quantitative analysis by immunodepletion of CENP-A nucleosomes showed that most of the CENP-C and approximately half the CENP-B took part in formation of the CENP-A/B/C chromatin complex. A kinetic study of the solubilization of CENPs showed that MNase digestion first released the CENP-A/B/C chromatin complex into the soluble fraction, and later removed CENP-B and CENP-C from the complex. This result suggests that CENP-A nucleosomes form a complex with CENP-B and CENP-C through interaction with DNA. On the basis of these results, we propose that the CENP-A/B/C chromatin complex is selectively formed on the I-type alpha-satellite array and constitutes the prekinetochore in HeLa cells.
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