ArticleMolecular and cellular biology2002
p53-dependent S-phase damage checkpoint and pronuclear cross talk in mouse zygotes with X-irradiated sperm.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 103 citations in OpenAlex.
- Sperm DNA integrity and male infertility: a narrative review and guide for the reproductive physicians.Translational andrology and urology · 2022Review
- Review
- Effects of space flight on sperm function and integrity: A systematic review.Frontiers in physiology · 2022Review
- Core Histones Are Constituents of the Perinuclear Theca of Murid Spermatozoa: An Assessment of Their Synthesis and Assembly during Spermiogenesis and Function after Gametic Fusion.International journal of molecular sciences · 2021Article
- Sperm Oxidative Stress during In Vitro Manipulation and Its Effects on Sperm Function and Embryo Development.Antioxidants (Basel, Switzerland) · 2021Review
- Genetic mechanisms of formation of radiation-induced instability of the genome and its transgenerational effects in the descendants of chronically irradiated individuals of Drosophila melanogaster.Radiation and environmental biophysics · 2020Article
- Article
- AMPK Activity Contributes to G2 Arrest and DNA Damage Decrease via p53/p21 Pathways in Oxidatively Damaged Mouse Zygotes.Frontiers in cell and developmental biology · 2020Article
- Spent embryo culture medium metabolites are related to the in vitro attachment ability of blastocysts.Scientific reports · 2018Article
- Unraveling the association between genetic integrity and metabolic activity in pre-implantation stage embryos.Scientific reports · 2016Article
- Oxidative stress-induced DNA damage of mouse zygotes triggers G2/M checkpoint and phosphorylates Cdc25 and Cdc2.Cell stress & chaperones · 2016Article
- Nuclear DNA fragmentation negatively affects zona binding competence of Y bearing mouse spermatozoa.Journal of assisted reproduction and genetics · 2013Article
- Chromosomal stability of second polar bodies in mouse embryos.Journal of assisted reproduction and genetics · 2013Article
- Mouse zygotes respond to severe sperm DNA damage by delaying paternal DNA replication and embryonic development.PloS one · 2013Article
- Semen abnormalities, sperm DNA damage and global hypermethylation in health workers occupationally exposed to ionizing radiation.PloS one · 2013Article
- Reduction in sperm aneuploidy levels in severe oligoasthenoteratospermic patients after medical therapy: a preliminary report.Asian journal of andrology · 2012Article
- Polycomb function during oogenesis is required for mouse embryonic development.Genes & development · 2012Article
- The number of spermatozoa collected with testicular sperm extraction is a novel predictor of intracytoplasmic sperm injection outcome in non-obstructive azoospermic patients.Asian journal of andrology · 2011Article
- Apoptosis and DNA damage in human spermatozoa.Asian journal of andrology · 2011Review
- Paternal levels of DNA damage in spermatozoa and maternal parity influence offspring mortality in an endangered ungulate.Proceedings. Biological sciences · 2010Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One difficulty in analyzing the damage response is that the effect of damage itself and that of cellular response are hard to distinguish in irradiated cells. In mouse zygotes, damage can be introduced by irradiated sperm, while damage response can be studied in the unirradiated maternal pronucleus. We have analyzed the p53-dependent damage responses in irradiated-sperm mouse zygotes and found that a p53-responsive reporter was efficiently activated in the female pronucleus. [(3)H]thymidine labeling experiments indicated that irradiated-sperm zygotes were devoid of G(1)/S arrest, but pronuclear DNA synthesis was suppressed equally in male and female pronuclei. p53(-/-) zygotes lacked this suppression, which was corrected by microinjection of glutathione S-transferase-p53 fusion protein. In contrast, p21(-/-) zygotes exhibited the same level of suppression upon fertilization by irradiated sperm. About a half of the 6-Gy-irradiated-sperm zygotes managed to synthesize a full DNA content by prolonging S phase, while the other half failed to do so. Regardless of the DNA content, all the zygotes cleaved to become two-cell-stage embryos. These results revealed the presence of p53-dependent pronuclear cross talk and a novel function of p53 in the S-phase DNA damage checkpoint of mouse zygotes.
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