ArticleMolecular and cellular biology2002
Partial reconstitution of human DNA mismatch repair in vitro: characterization of the role of human replication protein A.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 90 citations in OpenAlex.
- Action-At-A-Distance in DNA Mismatch Repair: Mechanistic Insights and Models for How DNA and Repair Proteins Facilitate Long-Range Communication.Biomolecules · 2024Review
- Exploring DNA Damage and Repair Mechanisms: A Review with Computational Insights.Biotech (Basel (Switzerland)) · 2024Review
- Article
- Unboxing the molecular modalities of mutagens in cancer.Environmental science and pollution research international · 2022Review
- OB-Folds and Genome Maintenance: Targeting Protein-DNA Interactions for Cancer Therapy.Cancers · 2021Review
- DNA mismatch repair in trinucleotide repeat instability.Science China. Life sciences · 2017Review
- Human DNA polymerase delta double-mutant D316A;E318A interferes with DNA mismatch repair in vitro.Nucleic acids research · 2017Article
- Mechanisms in E. coli and Human Mismatch Repair (Nobel Lecture).Angewandte Chemie (International ed. in English) · 2016Review
- Dynamic control of strand excision during human DNA mismatch repair.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Review
- Chromosomal directionality of DNA mismatch repair in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Phosphorylation of PCNA by EGFR inhibits mismatch repair and promotes misincorporation during DNA synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Single molecule studies of DNA mismatch repair.DNA repair · 2014Review
- Distinct structural alterations in proliferating cell nuclear antigen block DNA mismatch repair.Biochemistry · 2013Article
- The mechanism of mismatch repair and the functional analysis of mismatch repair defects in Lynch syndrome.Familial cancer · 2013Review
- The Werner syndrome protein promotes CAG/CTG repeat stability by resolving large (CAG)(n)/(CTG)(n) hairpins.The Journal of biological chemistry · 2012Article
- Repair-specific functions of replication protein A.The Journal of biological chemistry · 2012Article
- The Role of XPG in Processing (CAG)n/(CTG)n DNA Hairpins.Cell & bioscience · 2011Article
- Replication protein A: directing traffic at the intersection of replication and repair.Frontiers in bioscience (Landmark edition) · 2010Review
- Hyperphosphorylation of replication protein A in cisplatin-resistant and -sensitive head and neck squamous cell carcinoma cell lines.Head & neck · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
DNA mismatch repair (MMR) is a critical genome-stabilization system. However, the molecular mechanism of MMR in human cells remains obscure because many of the components have not yet been identified. Using a functional in vitro reconstitution system, this study identified three HeLa cell fractions essential for in vitro MMR. These fractions divide human MMR into two distinct stages: mismatch-provoked excision and repair synthesis. In vitro dissection of the MMR reaction and crucial intermediates elucidated biochemical functions of individual fractions in human MMR and identified hitherto unknown functions of human replication protein A (hRPA) in MMR. Thus, one fraction carries out nick-directed and mismatch-dependent excision; the second carries out DNA repair synthesis and DNA ligation; and the third provides hRPA, which plays multiple roles in human MMR by protecting the template DNA strand from degradation, enhancing repair excision, and facilitating repair synthesis. It is anticipated that further analysis of these fractions will identify additional MMR components and enable the complete reconstitution of the human MMR pathway with purified proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.