ArticleJournal of virology2002
Bipartite signal for genomic RNA dimerization in Moloney murine leukemia virus.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Review
- NMR Studies of Retroviral Genome Packaging.Viruses · 2020Review
- Rous Sarcoma Virus Genomic RNA Dimerization Capability In Vitro Is Not a Prerequisite for Viral Infectivity.Viruses · 2020Article
- Human T-cell leukemia virus type 1 Gag domains have distinct RNA-binding specificities with implications for RNA packaging and dimerization.The Journal of biological chemistry · 2018Article
- Retroviral RNA Dimerization: From Structure to Functions.Frontiers in microbiology · 2018Review
- RNA dimerization plays a role in ribosomal frameshifting of the SARS coronavirus.Nucleic acids research · 2013Article
- Femtomole SHAPE reveals regulatory structures in the authentic XMRV RNA genome.Journal of the American Chemical Society · 2011Article
- Structure of a conserved retroviral RNA packaging element by NMR spectroscopy and cryo-electron tomography.Journal of molecular biology · 2010Article
- An RNA structural switch regulates diploid genome packaging by Moloney murine leukemia virus.Journal of molecular biology · 2010Article
- Secondary structure of the mature ex virio Moloney murine leukemia virus genomic RNA dimerization domain.Journal of virology · 2010Article
- Molecular simulation studies of monovalent counterion-mediated interactions in a model RNA kissing loop.Journal of molecular biology · 2009Article
- Characterization of a natural heterodimer between MLV genomic RNA and the SD' retroelement generated by alternative splicing.RNA (New York, N.Y.) · 2007Article
- Structure of an RNA switch that enforces stringent retroviral genomic RNA dimerization.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Identification and characterization of critical cis-acting sequences within the yeast Ty1 retrotransposon.RNA (New York, N.Y.) · 2005Article
- Nonrandom dimerization of murine leukemia virus genomic RNAs.Journal of virology · 2004Article
- mRNA molecules containing murine leukemia virus packaging signals are encapsidated as dimers.Journal of virology · 2004Article
- Complementarity-directed RNA dimer-linkage promotes retroviral recombination in vivo.Nucleic acids research · 2004Article
- The dimer initiation sequence stem-loop of human immunodeficiency virus type 1 is dispensable for viral replication in peripheral blood mononuclear cells.Journal of virology · 2003Article
- A role for a novel 'trans-pseudoknot' RNA-RNA interaction in the functional dimerization of human telomerase.Genes & development · 2003Article
- Sequences downstream of the 5' splice donor site are required for both packaging and dimerization of human immunodeficiency virus type 1 RNA.Journal of virology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Retroviral virions each contain two identical genomic RNA strands that are stably but noncovalently joined in parallel near their 5' ends. For certain viruses, this dimerization has been shown to depend on a unique RNA stem-loop locus, called the dimer initiation site (DIS), that efficiently homodimerizes through a palindromic base sequence in its loop. Previous studies with Moloney murine leukemia virus (Mo-MuLV) identified two alternative DIS loci that can each independently support RNA dimerization in vitro but whose relative contributions are unknown. We now report that both of these loci contribute to the assembly of the Mo-MuLV dimer. Using targeted deletions, point mutagenesis, and antisense oligonucleotides, we found that each of the two stem-loops forms as predicted and contributes independently to dimerization in vitro through a mechanism involving autocomplementary interactions of its loop. Disruption of either DIS locus individually reduced both the yield and the thermal stability of the in vitro dimers, whereas disruption of both eliminated dimerization altogether. Similarly, the thermal stability of virion-derived dimers was impaired by deletion of both DIS elements, and point mutations in either element produced defects in viral replication that correlated with their effects on in vitro RNA dimerization. These findings support the view that in some retroviruses, dimer initiation and stability involve two or more closely linked DIS loci which together align the nascent dimer strands in parallel and in register.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.