ArticleThe Journal of physiology2002
Skeletal muscle L-type Ca(2+) current modulation in gamma1-deficient and wildtype murine myotubes by the gamma1 subunit and cAMP.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- From α1s splicing to γ1 function: A new twist in subunit modulation of the skeletal muscle L-type Ca2+ channel.The Journal of general physiology · 2022Article
- Calcium current modulation by the γ1 subunit depends on alternative splicing of CaV1.1.The Journal of general physiology · 2022Article
- Into the spotlight: RGK proteins in skeletal muscle.Cell calcium · 2021Review
- Stac3 has a direct role in skeletal muscle-type excitation-contraction coupling that is disrupted by a myopathy-causing mutation.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Apparent lack of physical or functional interaction between CaV1.1 and its distal C terminus.The Journal of general physiology · 2015Article
- Exogenous connexin43-expressing autologous skeletal myoblasts ameliorate mechanical function and electrical activity of the rabbit heart after experimental infarction.International journal of experimental pathology · 2015Article
- Ca(V)1.1: The atypical prototypical voltage-gated Ca²⁺ channel.Biochimica et biophysica acta · 2013Review
- The ß subunit of voltage-gated Ca2+ channels.Physiological reviews · 2010Review
- A critical GxxxA motif in the gamma6 calcium channel subunit mediates its inhibitory effect on Cav3.1 calcium current.The Journal of physiology · 2008Article
- Mutations in a Drosophila alpha2delta voltage-gated calcium channel subunit reveal a crucial synaptic function.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- gamma1-dependent down-regulation of recombinant voltage-gated Ca2+ channels.Cellular and molecular neurobiology · 2007Article
- Functional roles of the gamma subunit of the skeletal muscle DHP-receptor.Journal of muscle research and cell motility · 2006Review
- Altered inactivation of Ca2+ current and Ca2+ release in mouse muscle fibers deficient in the DHP receptor gamma1 subunit.The Journal of general physiology · 2004Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Modulation of the steady-state inactivation and current amplitude by the gamma1 subunit of the murine skeletal muscle L-type Ca(2+) channel were investigated using the whole-cell patch-clamp technique. Transient expression of the gamma1 subunit, but not of the gamma2 (stargazin) protein, in primary cultured myotubes from gamma1-deficient mice shifted the steady-state inactivation approximately -15 mV, thereby restoring wildtype (WT) steady-state inactivation and current amplitude. The increased Ca(2+) current amplitude in gamma1-deficient cells was abolished in myotubes from animals of 4 weeks and older whereas the positive shift in steady-state inactivation was independent of mouse age. Raising intracellular cAMP levels using the membrane-permeant analogue 8-Br-cAMP led to an increase in Ca(2+) current amplitude in WT cells to the level in gamma1-deficient myotubes. There was no effect on the current amplitude in gamma1-deficient cells or on the steady-state inactivation in either genotype. Rp-cAMPS, a competitive inhibitor of cAMP-dependent protein kinase, had no effect on the WT Ca(2+) current amplitude and steady-state inactivation, but diminished the current amplitude in gamma1-deficient myotubes without affecting the steady-state inactivation in these cells. These data show that the increased Ca(2+) influx in myotubes lacking the gamma1 subunit, due to right-shifted steady-state inactivation and increased L-type Ca(2+) current amplitude, is determined by the gamma1 subunit. The effect on current amplitude depends on the age of the mice and its cAMP-dependent modulation appears to be controlled by the gamma1 subunit.
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