ArticleThe Journal of physiology2002
Inward-rectifying anion channels are expressed in the epithelial cells of choroid plexus isolated from ClC-2 'knock-out' mice.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
9 citing papers in PubMed, 30 citations in OpenAlex.
- Choroid Plexus Aquaporins in CSF Homeostasis and the Glymphatic System: Their Relevance for Alzheimer's Disease.International journal of molecular sciences · 2023Review
- Research and progress on ClC‑2 (Review).Molecular medicine reports · 2017Review
- Idiopathic cerebrospinal fluid overproduction: case-based review of the pathophysiological mechanism implied in the cerebrospinal fluid production.Croatian medical journal · 2014Review
- Choroid plexus transport: gene deletion studies.Fluids and barriers of the CNS · 2011Article
- Inward-rectifier chloride currents in Reissner's membrane epithelial cells.Biochemical and biophysical research communications · 2010Article
- Ion channel diversity, channel expression and function in the choroid plexuses.Cerebrospinal fluid research · 2007Article
- Water and solute secretion by the choroid plexus.Pflugers Archiv : European journal of physiology · 2007Review
- Ion channel function of aquaporin-1 natively expressed in choroid plexus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Molecular mechanisms of cerebrospinal fluid production.Neuroscience · 2004Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Choroid plexus epithelial cells express inward-rectifying anion channels which have a high HCO(3)(-) permeability. These channels are thought to have an important role in the secretion of cerebrospinal fluid. The possible relationship between these channels and the ClC-2 Cl(-) channel was investigated in the present study. RT-PCR, using specific ClC-2 primers, amplified a 238 bp fragment of mRNA from rat choroid plexus, which was 99 % identical to the 5' sequence of rat ClC-2. A 2005 bp clone was isolated from a rat choroid plexus cDNA library using a probe for ClC-2. The clone showed greater than 99 % identity with the sequence of rat ClC-2. Inward-rectifying anion channels were observed in whole-cell recordings of choroid plexus epithelial cells isolated from ClC-2 knock-out mice. The mean inward conductance was 19.6 plus minus 3.6 nS (n = 8) in controls (3 heterozygote animals), and 22.5 plus minus 3.1 nS (n = 10) in three knock-out animals. The relative permeability of the conductances to I(-) and Cl(-) (P(I) : P(Cl)) was determined. I(-) was more permeant than Cl(-) in both heterozygotes (P(I):P(Cl) = 4.0 +/- 0.9, n = 3) and knock-out animals (P(I) : P(Cl) = 4.1 +/- 1.4, n = 3). These results indicate that rat choroid plexus expresses the ClC-2 variant that was originally reported in other tissues. ClC-2 does not contribute significantly to inward-rectifying anion conductance in mouse choroid plexus, which must therefore express a novel inward-rectifying anion channel.
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Registered trials
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