ArticleJournal of clinical microbiology2002
Application of a 5' nuclease assay for detection of Lawsonia intracellularis in fecal samples from pigs.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 59 citations in OpenAlex.
- Vaccination against Lawsonia intracellularis reduced tail-biting related behaviors in a commercial pig herd.Porcine health management · 2026Article
- Prevalence ofFrontiers in microbiology · 2026Article
- Vaccination Reduces Fecal Shedding and Improves Carcass Quality in Pigs with SubclinicalVaccines · 2025Article
- Establishment of a TaqMan Quantitative Real-Time PCR for DetectingVeterinary sciences · 2025Article
- Fecal PCR survey and genome analysis ofFrontiers in veterinary science · 2024Article
- Review of methods for the detection ofJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Review
- Development of a high-throughput real-time PCR system for detection of enzootic pathogens in pigs.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020Article
- Bacillus pumilus probiotic feed supplementation mitigates Lawsonia intracellularis shedding and lesions.Veterinary research · 2019Article
- A survey on the occurrence ofF1000Research · 2019Article
- Down-regulation of mechanisms involved in cell transport and maintenance of mucosal integrity in pigs infected with Lawsonia intracellularis.Veterinary research · 2014Article
- Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection.Acta veterinaria Scandinavica · 2010Article
- Human inflammatory bowel disease does not associate with Lawsonia intracellularis infection.BMC microbiology · 2006Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 5' nuclease assay was developed to detect Lawsonia intracellularis in porcine fecal samples. The specific probe and primers were chosen by using the 16S ribosomal DNA gene as a target. The 5' nuclease assay was used with a total of 204 clinical samples, and the results were compared to those of immunohistochemistry (IM) on ileal sections of the same animals. There was 91% agreement between the results of IM and the 5' nuclease assay. In the 5' nuclease assay, 111 (54%) of the pigs tested positive for L. intracellularis infection, with a mean cycle threshold (Ct) value of 27.2, whereas 98 (48%) of the pigs tested positive by IM. On average, the Ct and DeltaRn values for the positive samples were 27.2 (standard deviation [SD], 3.7) and 1.6 (SD, 0.7), respectively. A Ct value of 27.2 corresponds to a fecal excretion of approximately 10(7) L. intracellularis cells per g of feces. Furthermore, a total of 40 fecal samples derived from a herd known to be free from infection with L. intracellularis all tested negative, with a Ct value of 40. By using a Ct value of 36 as the cutoff limit, the detection limit of the assay was 1 L. intracellularis cell per PCR tube. In conclusion, the 5' nuclease assay that has been developed represents an applicable fast method for detection of L. intracellularis in fecal samples, with a sensitivity and specificity comparable to those of IM.
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