ArticleAmerican journal of physiology. Cell physiology2002
Heterologous desensitization of EGF receptors and PDGF receptors by sequestration in caveolae.
Article in American journal of physiology. Cell physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 70 citations in OpenAlex.
- Article
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- Hydrophobic matching controls the tilt and stability of the dimeric platelet-derived growth factor receptor (PDGFR) β transmembrane segment.The Journal of biological chemistry · 2012Article
- Article
- Endocytosis and spatial restriction of cell signaling.Molecular oncology · 2009Review
- Reciprocal regulation of the platelet-derived growth factor receptor-beta and G protein-coupled receptor kinase 5 by cross-phosphorylation: effects on catalysis.Molecular pharmacology · 2009Article
- Connexin hemichannel composition determines the FGF-1-induced membrane permeability and free [Ca2+]i responses.Molecular biology of the cell · 2008Article
- Plasma membrane domain organization regulates EGFR signaling in tumor cells.The Journal of cell biology · 2007Article
- Immunohistochemical study on caveolin-1alpha in regenerating process of tubular cells in gentamicin-induced acute tubular injury in rats.Virchows Archiv : an international journal of pathology · 2007Article
- Lipid rafts and caveolae in signaling by growth factor receptors.The open biochemistry journal · 2007Article
- Spatial segregation of transport and signalling functions between human endothelial caveolae and lipid raft proteomes.The Biochemical journal · 2006Article
- Relationships between EGFR signaling-competent and endocytosis-competent membrane microdomains.Molecular biology of the cell · 2005Article
- Distinct mechanisms determine the patterns of differential activation of H-Ras, N-Ras, K-Ras 4B, and M-Ras by receptors for growth factors or antigen.Molecular and cellular biology · 2004Article
- Fluorescence energy transfer reveals microdomain formation at physiological temperatures in lipid mixtures modeling the outer leaflet of the plasma membrane.Biophysical journal · 2003Article
- Regulation of raft-dependent endocytosis.Journal of cellular and molecular medicineReview
Corrections and comments
- Retraction · 2013-04-15Error in Image · Falsification/Fabrication of Data · Misconduct by Author ·
- Commented on by
- Commented on by
- RetractedRetraction.2013
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) receptors have been reported to signal via caveolin-containing membranes called caveolae. In contrast, others report that EGF and PDGF receptors are exclusively associated with caveolin-devoid membranes called rafts. Our subcellular fractionation and coimmunoprecipitation studies demonstrate that, in the absence of ligand, EGF and PDGF receptors are associated with rafts. However, in the presence of ligand, EGF and PDGF receptors transiently associate with caveolae. Surprisingly, pretreatment of cells with EGF prevents PDGF-dependent phosphorylation of PDGF receptors and extracellular signal-regulated kinase (ERK) 1/2 kinase activation. Furthermore, cells pretreated with PDGF prevent EGF-dependent phosphorylation of EGF receptors and ERK1/2 kinase activation. Radioligand binding studies demonstrate that incubation of cells with EGF or PDGF causes both EGF and PDGF receptors to be reversibly sequestered from the extracellular space. Experiments with methyl-beta-cyclodextrin, filipin, and antisense caveolin-1 demonstrate that sequestration of the receptors is dependent on cholesterol and caveolin-1. We conclude that ligand-induced stimulation of EGF or PDGF receptors can cause the heterologous desensitization of the other receptor by sequestration in cholesterol-rich, caveolin-containing membranes or caveolae.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.