ArticleThe Biochemical journal2002
The beta-appendages of the four adaptor-protein (AP) complexes: structure and binding properties, and identification of sorting nexin 9 as an accessory protein to AP-2.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Genome-Wide Association Approach Identified Novel Genetic Predictors of Heart Rate Response to β-Blockers.Journal of the American Heart Association · 2018Pooled it
- Multi-modal adaptor-clathrin contacts drive coated vesicle assembly.The EMBO journal · 2021Article
- Article
- HIP1R targets PD-L1 to lysosomal degradation to alter T cell-mediated cytotoxicity.Nature chemical biology · 2019Article
- Clever Cooperation: Interactions Between EspF and Host Proteins.Frontiers in microbiology · 2018Review
- Endocytosis, Metastasis and Beyond: Multiple Facets of SNX9.Trends in cell biology · 2017Review
- Depletion or over-expression of Sh3px1 results in dramatic changes in cell morphology.Biology open · 2015Article
- Membrane remodeling by the PX-BAR protein SNX18 promotes autophagosome formation.The Journal of cell biology · 2013Article
- The apoptotic engulfment protein Ced-6 participates in clathrin-mediated yolk uptake in Drosophila egg chambers.Molecular biology of the cell · 2012Article
- Functional analysis of interaction sites on the N-terminal domain of clathrin heavy chain.Traffic (Copenhagen, Denmark) · 2012Article
- Hotspots organize clathrin-mediated endocytosis by efficient recruitment and retention of nucleating resources.Traffic (Copenhagen, Denmark) · 2011Article
- E. coli secreted protein F promotes EPEC invasion of intestinal epithelial cells via an SNX9-dependent mechanism.Cellular microbiology · 2010Article
- SNX-BAR proteins in phosphoinositide-mediated, tubular-based endosomal sorting.Seminars in cell & developmental biology · 2010Review
- Adaptor protein-2 interaction with arrestin regulates GPCR recycling and apoptosis.Traffic (Copenhagen, Denmark) · 2009Article
- Out, in and back again: PtdIns(4,5)P(2) regulates cadherin trafficking in epithelial morphogenesis.The Biochemical journal · 2009Review
- The AP-2 adaptor beta2 appendage scaffolds alternate cargo endocytosis.Molecular biology of the cell · 2008Article
- Ever-expanding network of dynamin-interacting proteins.Molecular neurobiology · 2006Review
- Cell biology of membrane trafficking in human disease.International review of cytology · 2006Review
- SNX9 regulates dynamin assembly and is required for efficient clathrin-mediated endocytosis.Molecular biology of the cell · 2005Article
- The adaptor protein AP-4 as a component of the clathrin coat machinery: a morphological study.The Biochemical journal · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adaptor protein (AP) complexes are essential components for the formation of coated vesicles and the recognition of cargo proteins for intracellular transport. Each AP complex exposes two appendage domains with that function to bind regulatory accessory proteins in the cytosol. Secondary structure predictions, sequence alignments and CD spectroscopy were used to relate the beta-appendages of all human AP complexes to the previously published crystal structure of AP-2. The results suggested that the beta-appendages of AP-1, AP-2 and AP-3 have similar structures, consisting of two subdomains, whereas that of AP-4 lacks the inner subdomain. Pull-down and overlay assays showed partial overlap in the binding specificities of the beta-appendages of AP-1 and AP-2, whereas the corresponding domain of AP-3 displayed a unique binding pattern. That AP-4 may have a truncated, non-functional domain was indicated by its apparent inability to bind any proteins from cytosol. Of several novel beta-appendage-binding proteins detected, one that had affinity exclusively for AP-2 was identified as sorting nexin 9 (SNX9). SNX9, which contains a phox and an Src homology 3 domain, was found in large complexes and was at least partially associated with AP-2 in the cytosol. SNX9 may function to assist AP-2 in its role at the plasma membrane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.