ArticleThe Journal of experimental medicine2002
In vitro generation of interleukin 10-producing regulatory CD4(+) T cells is induced by immunosuppressive drugs and inhibited by T helper type 1 (Th1)- and Th2-inducing cytokines.
Article in The Journal of experimental medicine, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 386 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
386 citing papers in PubMed, 2 syntheses or guidelines pooled it, 1,152 citations in OpenAlex.
- Vitamin D supplementation in pregnant or breastfeeding women or young children for preventing asthma.The Cochrane database of systematic reviews · 2025Pooled it
- Effects of Vitamin D on Respiratory Function and Immune Status for Patients with Chronic Obstructive Pulmonary Disease (COPD): A Systematic Review and Meta-Analysis.Computational and mathematical methods in medicine · 2022Pooled it
- Serum and gene expression profile of cytokines following combination of yoga training and vitamin D supplementation in breast cancer survivors: a randomized controlled trial.BMC women's health · 2022Trial
- Transcriptome modulation by hydrocortisone in severe burn shock: ancillary analysis of a prospective randomized trial.Critical care (London, England) · 2017Trial
- Prenatal high-dose vitamin D3 supplementation has balanced effects on cord blood Th1 and Th2 responses.Nutrition journal · 2016Trial
- Effects of Systemically Administered Hydrocortisone on the Human Immunome.Scientific reports · 2016Trial
- Distinct endotypes of steroid-resistant asthma characterized by IL-17A(high) and IFN-γ(high) immunophenotypes: Potential benefits of calcitriol.The Journal of allergy and clinical immunology · 2015Trial
- Impact of vitamin D supplementation on arterial vasomotion, stiffness and endothelial biomarkers in chronic kidney disease patients.PloS one · 2014Trial
- Increased telomerase activity and vitamin D supplementation in overweight African Americans.International journal of obesity (2005) · 2012Trial
- Cytokines Associated with Activation of CD4International journal of molecular sciences · 2026Review
- Differential regulation of type 2 immunity by glucocorticoids.BMB reports · 2026Review
- Decreased 24-hour Parasympathetic Activity following Intracerebral Hemorrhage: A Key Factor Correlated with Adverse Perihematomal Edema and Poor Functional Outcomes.CNS neuroscience & therapeutics · 2026Article
- Baseline serum cortisol as a prognostic biomarker for immune checkpoint inhibitor therapy in advanced gastric cancer.Frontiers in oncology · 2026Article
- The 1α,25-dihydroxyvitamin D3 modulates T cell activation and immune checkpoint pathways in human T cells.Frontiers in immunology · 2026Article
- Myeloid cell expression of the VDR and parathyroid hormone control the host response to influenza infection in mice.Frontiers in immunology · 2026Article
- The Potential of β-Synuclein-Specific Regulatory T Cell Therapy as a Treatment for Progressive Multiple Sclerosis.International journal of molecular sciences · 2025Review
- Deciphering immune cell interaction aberrations in necrotizing enterocolitis: the regulatory role of DHRS7.Frontiers in immunology · 2025Article
- Comparison of the role of vitamin D in normal organs and those affected by COVID-19.International journal of medical sciences · 2025Review
- Impacts of tacrolimus and glucocorticoids on peripheral blood T and B lymphocyte subsets in myasthenia gravis.Frontiers in immunology · 2025Article
- Impact of peripheral lymphocyte subsets on prognosis for patients after acute ischemic stroke: A potential disease prediction model approach.CNS neuroscience & therapeutics · 2024Article
326 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We show that a combination of the immunosuppressive drugs, vitamin D3 and Dexamethasone, induced human and mouse naive CD4(+) T cells to differentiate in vitro into regulatory T cells. In contrast to the previously described in vitro derived CD4(+) T cells, these cells produced only interleukin (IL)-10, but no IL-5 and interferon (IFN)-gamma, and furthermore retained strong proliferative capacity. The development of these IL-10-producing cells was enhanced by neutralization of the T helper type 1 (Th1)- and Th2-inducing cytokines IL-4, IL-12, and IFN-gamma. These immunosuppressive drugs also induced the development of IL-10-producing T cells in the absence of antigen-presenting cells, with IL-10 acting as a positive autocrine factor for these T cells. Furthermore, nuclear factor (NF)-kappaB and activator protein (AP)-1 activities were inhibited in the IL-10-producing cells described here as well as key transcription factors involved in Th1 and Th2 subset differentiation. The regulatory function of these in vitro generated IL-10-producing T cells was demonstrated by their ability to prevent central nervous system inflammation, when targeted to the site of inflammation, and this function was shown to be IL-10 dependent. Generating homogeneous populations of IL-10-producing T cells in vitro will thus facilitate the use of regulatory T cells in immunotherapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.