ArticleThe Journal of cell biology2002
High RhoA activity maintains the undifferentiated mesenchymal cell phenotype, whereas RhoA down-regulation by laminin-2 induces smooth muscle myogenesis.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 87 citations in OpenAlex.
- Analyzing soluble and lung smooth muscle laminin isoform expression in guinea pigs exhibiting various allergic response phenotypes.Frontiers in immunology · 2026Article
- Geranylgeraniol prevents zoledronic acid-mediated reduction of viable mesenchymal stem cells via induction of Rho-dependent YAP activation.Royal Society open science · 2021Article
- Article
- Molecular and Biomechanical Clues From Cardiac Tissue Decellularized Extracellular Matrix Drive Stromal Cell Plasticity.Frontiers in bioengineering and biotechnology · 2020Article
- Stochastic Dynamics of Gene Switching and Energy Dissipation for Gene Expression.Frontiers in genetics · 2020Article
- Cytoplasmic sequestration of the RhoA effector mDiaphanous1 by Prohibitin2 promotes muscle differentiation.Scientific reports · 2019Article
- Sustained Activation of Rho GTPases Promotes a Synthetic Pulmonary Artery Smooth Muscle Cell Phenotype in Neprilysin Null Mice.Arteriosclerosis, thrombosis, and vascular biology · 2018Article
- RhoA/ROCK inhibition improves the beneficial effects of glucocorticoid treatment in dystrophic muscle: implications for stem cell depletion.Human molecular genetics · 2017Article
- Role of EMT in Metastasis and Therapy Resistance.Journal of clinical medicine · 2016Review
- The transcription factor serum response factor stimulates axon regeneration through cytoplasmic localization and cofilin interaction.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- p190-B RhoGAP regulates the functional composition of the mesenchymal microenvironment.Leukemia · 2013Article
- RhoA-Rho kinase and platelet-activating factor stimulation of ovine foetal pulmonary vascular smooth muscle cell proliferation.Cell proliferation · 2013Article
- RhoA mediates defective stem cell function and heterotopic ossification in dystrophic muscle of mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- MicroRNA-148a promotes myogenic differentiation by targeting the ROCK1 gene.The Journal of biological chemistry · 2012Article
- Motility, survival, and proliferation.Comprehensive Physiology · 2012Review
- Mechanisms of remodeling in asthmatic airways.Journal of allergy · 2012Article
- The extracellular matrix in development and morphogenesis: a dynamic view.Developmental biology · 2010Review
- Small functional groups for controlled differentiation of hydrogel-encapsulated human mesenchymal stem cells.Nature materials · 2008Article
- The role of FoxO in the regulation of metabolism.Oncogene · 2008Review
- Embryological origin of airway smooth muscle.Proceedings of the American Thoracic Society · 2008Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Round embryonic mesenchymal cells have the potential to differentiate into smooth muscle (SM) cells upon spreading/elongation (Yang, Y., K.C. Palmer, N. Relan, C. Diglio, and L. Schuger. 1998. Development. 125:2621-2629; Yang, Y., N.K. Relan, D.A. Przywara, and L. Schuger. 1999. Development. 126:3027-3033; Yang, Y., S. Beqaj, P. Kemp, I. Ariel, and L. Schuger. 2000. J. Clin. Invest. 106:1321-1330). In the developing lung, this process is stimulated by peribronchial accumulation of laminin (LN)-2 (Relan, N.K., Y. Yang, S. Beqaj, J.H. Miner, and L. Schuger. 1999. J. Cell Biol. 147:1341-1350). Here we show that LN-2 stimulates bronchial myogenesis by down-regulating RhoA activity. Immunohistochemistry, immunoblotting, and reverse transcriptase-PCR indicated that RhoA, a small GTPase signaling protein, is abundant in undifferentiated embryonic mesenchymal cells and that its levels decrease along with SM myogenesis. Functional studies using agonists and antagonists of RhoA activation and dominant positive and negative plasmid constructs demonstrated that high RhoA activity was required to maintain the round undifferentiated mesenchymal cell phenotype. This was in part achieved by restricting the localization of the myogenic transcription factor serum response factor (SRF) mostly to the mesenchymal cell cytoplasm. Upon spreading on LN-2 but not on other main components of the extracellular matrix, the activity and level of RhoA decreased rapidly, resulting in translocation of SRF to the nucleus. Both cell elongation and SRF translocation were prevented by overexpression of dominant positive RhoA. Once the cells underwent SM differentiation, up-regulation of RhoA activity induced rather than inhibited SM gene expression. Therefore, our studies suggest a novel mechanism whereby LN-2 and RhoA modulate SM myogenesis.
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