ArticleGenes & development2002
Calcineurin-dependent regulation of Crz1p nuclear export requires Msn5p and a conserved calcineurin docking site.
Article in Genes & development, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 116 citations in OpenAlex.
- Phosphate-dependent nuclear export via a non-classical NES class recognized by exportin Msn5.Nature communications · 2025Article
- The CRZ1 transcription factor in plant fungi: regulation mechanism and impact on pathogenesis.Molecular biology reports · 2024Review
- Calcineurin stimulation by Cnb1p overproduction mitigates protein aggregation and α-synuclein toxicity in a yeast model of synucleinopathy.Cell communication and signaling : CCS · 2023Article
- Updating Insights into the Regulatory Mechanisms of Calcineurin-Activated Transcription Factor Crz1 in Pathogenic Fungi.Journal of fungi (Basel, Switzerland) · 2022Review
- CRZ1 transcription factor is involved in cell survival, stress tolerance, and virulence in fungi.Journal of biosciences · 2022Review
- Characterization of nuclear pore complex targeting domains in Pom152 in Saccharomyces cerevisiae.Biology open · 2021Article
- A functionally divergent intrinsically disordered region underlying the conservation of stochastic signaling.PLoS genetics · 2021Article
- Karyopherin Msn5 is involved in a novel mechanism controlling the cellular level of cell cycle regulators Cln2 and Swi5.Cell cycle (Georgetown, Tex.) · 2019Article
- Casein Kinase I Isoform Hrr25 Is a Negative Regulator of Haa1 in the Weak Acid Stress Response Pathway in Saccharomyces cerevisiae.Applied and environmental microbiology · 2017Article
- Elucidation of the calcineurin-Crz1 stress response transcriptional network in the human fungal pathogen Cryptococcus neoformans.PLoS genetics · 2017Article
- Aspergillus fumigatus virulence through the lens of transcription factors.Medical mycology · 2017Review
- Dual control by Cdk1 phosphorylation of the budding yeast APC/C ubiquitin ligase activator Cdh1.Molecular biology of the cell · 2016Article
- Article
- Regulation of the Na+/K+-ATPase Ena1 Expression by Calcineurin/Crz1 under High pH Stress: A Quantitative Study.PloS one · 2016Article
- Short linear motifs - ex nihilo evolution of protein regulation.Cell communication and signaling : CCS · 2015Review
- Negative feedback regulation of calcineurin-dependent Prz1 transcription factor by the CaMKK-CaMK1 axis in fission yeast.Nucleic acids research · 2014Article
- Nuclear localization of Haa1, which is linked to its phosphorylation status, mediates lactic acid tolerance in Saccharomyces cerevisiae.Applied and environmental microbiology · 2014Article
- Calcineurin-Crz1 signaling in lower eukaryotes.Eukaryotic cell · 2014Review
- A calcineurin-dependent switch controls the trafficking function of α-arrestin Aly1/Art6.The Journal of biological chemistry · 2013Article
- Genetic bypass of Aspergillus nidulans crzA function in calcium homeostasis.G3 (Bethesda, Md.) · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Calcineurin, a conserved Ca(2+)/calmodulin-regulated protein phosphatase, plays a crucial role in Ca(2+) signaling in a wide variety of cell types. In Saccharomyces cerevisiae, calcineurin positively regulates transcription in response to stress by dephosphorylating the transcription factor Crz1p/Tcn1p. Dephosphorylation promotes Crz1p nuclear localization in part by increasing the efficiency of its nuclear import. In this work, we show that calcineurin-dependent dephosphorylation of Crz1p also down-regulates its nuclear export. Using a genetic approach, we identify Msn5p as the exportin for Crz1p. In addition, we define the Crz1p nuclear export signal (NES) and show that it interacts with Msn5p in a phosphorylation-dependent manner. This indicates that calcineurin regulates Crz1p nuclear export by dephosphorylating and inactivating its NES. Finally, we define a motif in Crz1p, PIISIQ, similar to the PxIxIT docking site for calcineurin on the mammalian transcription factor NFAT, that mediates the in vivo interaction between calcineurin and Crz1p and is required for calcineurin-dependent regulation of Crz1p nuclear export and activity. Therefore, in yeast as in mammals, a docking site is required to target calcineurin to its substrate such that it can dephosphorylate it efficiently.
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