ReviewMolecular biotechnology2002
Characteristics and applications of nucleic acid sequence-based amplification (NASBA).
Review in Molecular biotechnology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
97 citing papers in PubMed, 293 citations in OpenAlex.
- Hybridization-Based Electrochemical Biosensors for RNA Detection: Key Concepts and Insights.Sensors and actuators. B, Chemical · 2026Article
- CRISPR/Cas12a-assisted visual and on-site detection of porcine circovirus type 2.BMC veterinary research · 2026Article
- Point-of-Care Diagnostic Technologies for Antimicrobial Resistance: Principles, Platforms, Clinical Impact, and Future Directions.Diagnostics (Basel, Switzerland) · 2026Review
- Isothermal nucleic acid amplification techniques: A comprehensive overview on their principle & applications as alternative to PCR.Practical laboratory medicine · 2026Review
- Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of High-Burden Viral Diseases in Resource-Limited Countries.Pathogens (Basel, Switzerland) · 2026Review
- Overcoming Challenges in Avian Influenza Diagnosis: The Role of Surface-Enhanced Raman Spectroscopy in Poultry Health Monitoring.Veterinary sciences · 2025Review
- Next-Generation Nucleic Acid-Based Diagnostics for Viral Pathogens: Lessons Learned from the SARS-CoV-2 Pandemic.Microorganisms · 2025Review
- Advances in Epstein-Barr Virus Detection: From Traditional Methods to Modern Technologies.Viruses · 2025Review
- Rapid Amplification and Detection of Single-Stranded Nucleic Acids for Point-of-Care Diagnosis.Small methods · 2025Review
- Cleavable energy transfer labeled oligonucleotide probe for enhanced isothermal amplification detection and nano digital chip-based readout.Nanoscale · 2025Article
- Isothermal Amplification Techniques: An Emerging Tool for On-Site Detection of Phytopathogens in Field Conditions.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Progress in the application of isothermal amplification technology in the diagnosis of infectious diseases.Frontiers in microbiology · 2025Review
- The Evolution of Nucleic Acid-Based Diagnosis Methods from the (pre-)CRISPR to CRISPR era and the Associated Machine/Deep Learning Approaches in Relevant RNA Design.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections.Clinical microbiology reviews · 2024Review
- CRISPR-Cas13a-Based Lateral Flow Assay for Detection of Bovine Leukemia Virus.Animals : an open access journal from MDPI · 2024Article
- Loop-Mediated Isothermal Amplification Assay for the Detection of Citrus Canker Causing Bacterial Variant,International journal of molecular sciences · 2024Article
- AMPLON: Amplifying DNA with Multiarm Priming and Looping Optimization of Nucleic Acid.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- PACRAT: pathogen detection with aptamer-observed cascaded recombinase polymerase amplification-in vitro transcription.RNA (New York, N.Y.) · 2024Article
- An evaluation of nucleic acid-based molecular methods for the detection of plant viruses: a systematic review.Virusdisease · 2024Review
- Detection method for reverse transcription recombinase-aided amplification of avian influenza virus subtypes H5, H7, and H9.BMC veterinary research · 2024Article
37 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleic acid sequence-based amplification (NASBA) is a sensitive, isothermal, transcription-based amplification system specifically designed for the detection of RNA targets. In some NASBA systems, DNA is also amplified though very inefficiently and only in the absence of the corresponding RNA target or in case of an excess (>1,000-fold) of target DNA over RNA. As NASBA is primer-dependent and amplicon detection is based on probe binding, primer and probe design rules are included. An overview of various target nucleic acids that have been amplified successfully using NASBA is presented. For the isolation of nucleic acids prior to NASBA, the "Boom" method, based on the denaturing properties of guanidine isothiocyanate and binding of nucleic acid to silica particles, is preferred. Currently, electro-chemiluminescence (ECL) is recommended for the detection of the amplicon at the end of amplification. In the near future, molecular beacons will be introduced enabling "real-time detection," i.e., amplicon detection during amplification. Quantitative HIV-1 NASBA and detection of up to 48 samples can then be performed in only 90 min.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.