ArticleTransgenic research2002
Expression of the high capacity calcium-binding domain of calreticulin increases bioavailable calcium stores in plants.
Article in Transgenic research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 84 citations in OpenAlex.
- Article
- Cold-induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in rice.The EMBO journal · 2023Article
- Article
- Revitalization of small millets for nutritional and food security by advanced genetics and genomics approaches.Frontiers in genetics · 2022Review
- HaCRT1 ofFrontiers in plant science · 2020Article
- Plasmodesmata Conductivity Regulation: A Mechanistic Model.Plants (Basel, Switzerland) · 2019Review
- Article
- Calcium Biofortification: Three Pronged Molecular Approaches for Dissecting Complex Trait of Calcium Nutrition in Finger Millet (Frontiers in plant science · 2016Review
- Overexpression of a Triticum aestivum Calreticulin gene (TaCRT1) Improves Salinity Tolerance in Tobacco.PloS one · 2015Article
- The ER quality control and ER associated degradation machineries are vital for viral pathogenesis.Frontiers in plant science · 2014Review
- Callose homeostasis at plasmodesmata: molecular regulators and developmental relevance.Frontiers in plant science · 2014Article
- Loss of all three calreticulins, CRT1, CRT2 and CRT3, causes enhanced sensitivity to water stress in Arabidopsis.Plant cell reports · 2013Article
- Ectopic expression of a maize calreticulin mitigates calcium deficiency-like disorders in sCAX1-expressing tobacco and tomato.Plant molecular biology · 2012Article
- An ER-targeted calcium-binding peptide confers salt and drought tolerance mediated by CIPK6 in Arabidopsis.Planta · 2012Article
- Review
- Basal signaling regulates plant growth and development.Plant physiology · 2010Article
- Article
- Helper component-proteinase (HC-Pro) protein of Papaya ringspot virus interacts with papaya calreticulin.Molecular plant pathology · 2010Article
- Calreticulin expression and localization in plant cells during pollen-pistil interactions.Planta · 2009Article
- A plant-specific calreticulin is a key retention factor for a defective brassinosteroid receptor in the endoplasmic reticulum.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Modulation of cytosolic calcium levels in both plants and animals is achieved by a system of Ca2+-transport and storage pathways that include Ca2+ buffering proteins in the lumen of intracellular compartments. To date, most research has focused on the role of transporters in regulating cytosolic calcium. We used a reverse genetics approach to modulate calcium stores in the lumen of the endoplasmic reticulum. Our goals were two-fold: to use the low affinity, high capacity Ca2+ binding characteristics of the C-domain of calreticulin to selectively increase Ca2+ storage in the endoplasmic reticulum, and to determine if those alterations affected plant physiological responses to stress. The C-domain of calreticulin is a highly acidic region that binds 20-50 moles of Ca2+ per mole of protein and has been shown to be the major site of Ca2+ storage within the endoplasmic reticulum of plant cells. A 377-bp fragment encoding the C-domain and ER retention signal from the maize calreticulin gene was fused to a gene for the green fluorescent protein and expressed in Arabidopsis under the control of a heat shock promoter. Following induction on normal medium, the C-domain transformants showed delayed loss of chlorophyll after transfer to calcium depleted medium when compared to seedlings transformed with green fluorescent protein alone. Total calcium measurements showed a 9-35% increase for induced C-domain transformants compared to controls. The data suggest that ectopic expression of the calreticulin C-domain increases Ca2+ stores, and that this Ca2+ reserve can be used by the plant in times of stress.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.