ArticleRNA (New York, N.Y.)2002
Mutational analysis of a plant branchpoint and polypyrimidine tract required for constitutive splicing of a mini-exon.
Article in RNA (New York, N.Y.), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 41 citations in OpenAlex.
- Arabidopsis exoribonuclease USB1 interacts with the PPR-domain protein SOAR1 to negatively regulate abscisic acid signaling.Journal of experimental botany · 2020Article
- Identification and Analysis of Micro-Exon Genes in the Rice Genome.International journal of molecular sciences · 2019Article
- An intragenic mutagenesis strategy in Physcomitrella patens to preserve intron splicing.Scientific reports · 2017Article
- Alternative splicing of mini-exons in the Arabidopsis leaf rust receptor-like kinase LRK10 genes affects subcellular localisation.Plant cell reports · 2015Article
- Complexity of the alternative splicing landscape in plants.The Plant cell · 2013Review
- A mutation screening platform for rapeseed (Brassica napus L.) and the detection of sinapine biosynthesis mutants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- The spliceosome-activating complex: molecular mechanisms underlying the function of a pleiotropic regulator.Frontiers in plant science · 2012Article
- Deciphering the plant splicing code: experimental and computational approaches for predicting alternative splicing and splicing regulatory elements.Frontiers in plant science · 2012Article
- Factors affecting splicing strength of yeast genes.Comparative and functional genomics · 2011Article
- Genomic characterization and linkage mapping of the apple allergen genes Mal d 2 (thaumatin-like protein) and Mal d 4 (profilin).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Genomic cloning and linkage mapping of the Mal d 1 (PR-10) gene family in apple (Malus domestica).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2005Article
- Comparative sequence analysis of the region harboring the hardness locus in barley and its colinear region in rice.Plant physiology · 2004Article
- The Elm1 (ZmHy2) gene of maize encodes a phytochromobilin synthase.Plant physiology · 2004Article
- Analysis of splice donor and acceptor function in a novel Ac-based gene trap construct.Planta · 2004Article
- Determinants of plant U12-dependent intron splicing efficiency.The Plant cell · 2004Article
- Article
- Requirements for intron-mediated enhancement of gene expression in Arabidopsis.RNA (New York, N.Y.) · 2002Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The branchpoint sequence and associated polypyrimidine tract are firmly established splicing signals in vertebrates. In plants, however, these signals have not been characterized in detail. The potato invertase mini-exon 2 (9 nt) requires a branchpoint sequence positioned around 50 nt upstream of the 5' splice site of the neighboring intron and a U11 element found adjacent to the branchpoint in the upstream intron (Simpson et al., RNA, 2000, 6:422-433). Utilizing the sensitivity of this plant splicing system, these elements have been characterized by systematic mutation and analysis of the effect on inclusion of the mini-exon. Mutation of the branchpoint sequence in all possible positions demonstrated that branchpoints matching the consensus, CURAY, were most efficient at supporting splicing. Branchpoint sequences that differed from this consensus were still able to permit mini-exon inclusion but at greatly reduced levels. Mutation of the downstream U11 element suggested that it functioned as a polypyrimidine tract rather than a UA-rich element, common to plant introns. The minimum sequence requirement of the polypyrimidine tract for efficient splicing was two closely positioned groups of uridines 3-4 nt long (<6 nt apart) that, within the context of the mini-exon system, required being close (<14 nt) to the branchpoint sequence. The functional characterization of the branchpoint sequence and polypyrimidine tract defines these sequences in plants for the first time, and firmly establishes polypyrimidine tracts as important signals in splicing of at least some plant introns.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.