ArticleRNA (New York, N.Y.)2002
The Brome mosaic virus subgenomic promoter hairpin is structurally similar to the iron-responsive element and functionally equivalent to the minus-strand core promoter stem-loop C.
Article in RNA (New York, N.Y.), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 42 citations in OpenAlex.
- Characterization of Variant RNAs Encapsidated during Bromovirus Infection by High-Throughput Sequencing.Pathogens (Basel, Switzerland) · 2024Article
- Positive strand RNA viruses differ in the constraints they place on the folding of their negative strand.RNA (New York, N.Y.) · 2022Article
- Role of an RNA pseudoknot involving the polyA tail in replication of Pepino mosaic potexvirus and related plant viruses.Scientific reports · 2022Article
- Structural characterization of the ANTAR antiterminator domain bound to RNA.Nucleic acids research · 2022Article
- Review
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- On the conformational stability of the smallest RNA kissing complexes maintained through two G·C base pairs.Biochemical and biophysical research communications · 2017Article
- Co-infection with two strains of Brome mosaic bromovirus reveals common RNA recombination sites in different hosts.Virus evolution · 2015Article
- Structural and thermodynamic signatures that define pseudotriloop RNA hairpins.RNA (New York, N.Y.) · 2013Article
- Influence of genome-scale RNA structure disruption on the replication of murine norovirus--similar replication kinetics in cell culture but attenuation of viral fitness in vivo.Nucleic acids research · 2013Article
- The subgenomic promoter of brome mosaic virus folds into a stem-loop structure capped by a pseudo-triloop that is structurally similar to the triloop of the genomic promoter.RNA (New York, N.Y.) · 2012Article
- Review
- Antiviral strategies to control calicivirus infections.Antiviral research · 2010Review
- Bioinformatic and physical characterizations of genome-scale ordered RNA structure in mammalian RNA viruses.Journal of virology · 2008Article
- Bioinformatic and functional analysis of RNA secondary structure elements among different genera of human and animal caliciviruses.Nucleic acids research · 2008Article
- Thermodynamics and NMR studies on Duck, Heron and Human HBV encapsidation signals.Nucleic acids research · 2007Article
- Characterization of a novel 5' subgenomic RNA3a derived from RNA3 of Brome mosaic bromovirus.Journal of virology · 2006Article
- Analyses of subgenomic promoters of Hibiscus chlorotic ringspot virus and demonstration of 5' untranslated region and 3'-terminal sequences functioning as subgenomic promoters.Journal of virology · 2006Article
- Solution structure of the apical stem-loop of the human hepatitis B virus encapsidation signal.Nucleic acids research · 2006Article
- Mutual interference between genomic RNA replication and subgenomic mRNA transcription in brome mosaic virus.Journal of virology · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the Bromoviridae family of plant viruses, trinucleotide hairpin loops play an important role in RNA transcription. Recently, we reported that Brome mosaic virus (BMV) subgenomic (sg) transcription depended on the formation of an unusual triloop hairpin. By native gel electrophoresis, enzymatic structure probing, and NMR spectroscopy it is shown here that in the absence of viral replicase the hexanucleotide loop 5'C1AUAG5A3' of this RNA structure can adopt a pseudo trinucleotide loop conformation by transloop base pairing between C1 and G5. By means of in vitro replication assays using partially purified BMV RNA-dependent RNA polymerase (RdRp) it was found that other base pairs contribute to sg transcription, probably by stabilizing the formation of this pseudo triloop, which is proposed to be the primary element recognized by the viral replicase. The BMV pseudo triloop structure strongly resembles iron-responsive elements (IREs) in cellular messenger RNAs and may represent a general protein-binding motif. In addition, in vitro replication assays showed that the BMV sg hairpin is functionally equivalent to the minus-strand core promoter hairpin stem-loop C at the 3' end of BMV RNAs. Replacement of the sg hairpin by stem-loop C yielded increased sg promoter activity whereas replacement of stem-loop C by the sg hairpin resulted in reduced minus-strand promoter activity. We conclude that AUA triloops represent the common motif in the BMV sg and minus-strand promoters required for recruitment of the viral replicase. Additional sequence elements of the minus-strand promoter are proposed to direct the RdRp to the initiation site at the 3' end of the genomic RNA.
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