ArticleSexually transmitted infections2002
Polymerase chain reaction for diagnosis of genital herpes in a genitourinary medicine clinic.
Article in Sexually transmitted infections, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
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Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 80 citations in OpenAlex.
- Sexually transmitted diseases treatment guidelines, 2015.MMWR. Recommendations and reports : Morbidity and mortality weekly report. Recommendations and reports · 2015Guideline
- Genital herpes.Sexually transmitted infections · 2006Guideline
- Diagnosis of genital herpes by real time PCR in routine clinical practice.Sexually transmitted infections · 2004Trial
- Article
- Southern African HIV Clinicians Society 2022 guideline for the management of sexually transmitted infections: Moving towards best practice.Southern African journal of HIV medicine · 2022Article
- Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques.Infectious disease reports · 2021Review
- Comparison of Herpes Simplex Virus PCR with Culture for Virus Detection in Multisource Surface Swab Specimens from Neonates.Journal of clinical microbiology · 2018Article
- Development and evaluation of the quantitative real-time PCR assay in detection and typing of herpes simplex virus in swab specimens from patients with genital herpes.International journal of clinical and experimental medicine · 2015Article
- Diagnosis of genital herpes simplex virus infection in the clinical laboratory.Virology journal · 2014Review
- Herpes simplex encephalitis in Iceland 1987-2011.SpringerPlus · 2014Article
- Comparison of Simplexa HSV 1 & 2 PCR with culture, immunofluorescence, and laboratory-developed TaqMan PCR for detection of herpes simplex virus in swab specimens.Journal of clinical microbiology · 2013Article
- Herpes simplex virus infection in pregnancy and in neonate: status of art of epidemiology, diagnosis, therapy and prevention.Virology journal · 2009Review
- Genital herpes and its management.BMJ (Clinical research ed.) · 2007Review
- Herpes simplex virus type 2: epidemiology and management options in developing countries.Sexually transmitted infections · 2007Review
- Diagnostics for herpes simplex virus: is PCR the new gold standard?Molecular diagnosis & therapy · 2006Review
- Detection, quantification and genotyping of Herpes Simplex Virus in cervicovaginal secretions by real-time PCR: a cross sectional survey.Virology journal · 2005Article
- The laboratory diagnosis of herpes simplex virus infections.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2005Article
- Longitudinal study of genital infection by herpes simplex virus type 1 in Western Scotland over 15 years.BMJ (Clinical research ed.) · 2002Article
- Using the evidence base on genital herpes: optimising the use of diagnostic tests and information provision.Sexually transmitted infections · 2002Review
- Herpes Simplex Virus 1 and 2 in Herpes Genitalis: A Polymerase Chain Reaction-Based Study from Kerala.Indian journal of dermatologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPolymerase chain reaction (PCR) has well established advantages over culture for diagnosis of herpes viruses, but its technical complexity has limited its widespread application. However, recent methodological advances have rendered PCR more applicable to routine practice.
aimTo compare automated PCR with viral culture for diagnosis of genital herpes.
methodsWe studied 236 patients presenting with clinical features suggestive of genital herpes at an inner city genitourinary medicine clinic. Two swabs were taken from each patient. Cell culture and typing were performed by standard methods. Automated PCR was performed using the LightCycler instrument and the infecting viral type was determined by restriction endonuclease digestion of amplicons.
results109 patients (46%) had a positive test for herpes simplex virus (HSV). In 88, both PCR and culture were positive; in 21 PCR only was positive. With both detection methods, lesion duration and morphology were associated with HSV detection. Compared with culture alone, use of PCR increased sensitivity by 13.3% in specimens from vesicular lesions, by 27.4% from ulcerative lesions, and by 20.0% from crusting lesions.
conclusionsWe advocate adoption of automated PCR as an efficient HSV detection and typing method for diagnosis of genital herpes in routine clinical practice. PCR allowed rapid laboratory confirmation of the diagnosis and increased the overall HSV detection rate by 24%.
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