ArticleApplied and environmental microbiology2002
Quantitative detection of microbial genes by using DNA microarrays.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- An Ultrasensitive Electrochemical Biosensor for Nucleic Acid Detection Based on Silver Nanoflower-Stem-Loop Probes.Sensors (Basel, Switzerland) · 2026Article
- The Potential Correlation Between Bacterial Sporulation and the Characteristic Flavor of Chinese Maotai Liquor.Frontiers in microbiology · 2018Article
- Microbial degradation of petrochemical waste-polycyclic aromatic hydrocarbons.Bioresources and bioprocessing · 2017Article
- A Parametric Survival Model When a Covariate is Subject to Left-Censoring.Journal of biometrics & biostatistics · 2012Article
- Use of functional gene arrays for elucidating in situ biodegradation.Frontiers in microbiology · 2012Article
- Adaptation of methanogenic communities to the cofermentation of cattle excreta and olive mill wastes at 37 degrees C and 55 degrees C.Applied and environmental microbiology · 2010Article
- Development of a common oligonucleotide reference standard for microarray data normalization and comparison across different microbial communities.Applied and environmental microbiology · 2010Article
- Large scale multiplex PCR improves pathogen detection by DNA microarrays.BMC microbiology · 2009Article
- Transcriptome analysis of Lactococcus lactis in coculture with Saccharomyces cerevisiae.Applied and environmental microbiology · 2008Article
- Unravelling microbial communities with DNA-microarrays: challenges and future directions.Microbial ecology · 2007Review
- Current molecular techniques for the detection of microbial pathogens.Science progress · 2007Review
- Accurately quantifying low-abundant targets amid similar sequences by revealing hidden correlations in oligonucleotide microarray data.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Microarray applications in microbial ecology research.Microbial ecology · 2006Review
- Microarray-based analysis of subnanogram quantities of microbial community DNAs by using whole-community genome amplification.Applied and environmental microbiology · 2006Article
- Potential of a 16S rRNA-based taxonomic microarray for analyzing the rhizosphere effects of maize on Agrobacterium spp. and bacterial communities.Applied and environmental microbiology · 2006Article
- Development and applications of microbial ecogenomic indicators for monitoring water quality: report of a workshop assessing the state of the science, research needs and future directions.Environmental monitoring and assessment · 2006Article
- Detection of genes involved in biodegradation and biotransformation in microbial communities by using 50-mer oligonucleotide microarrays.Applied and environmental microbiology · 2004Article
- Fingerprinting diazotroph communities in the Chesapeake Bay by using a DNA macroarray.Applied and environmental microbiology · 2004Article
- Metagenomic profiling: microarray analysis of an environmental genomic library.Applied and environmental microbiology · 2003Article
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Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To quantify target genes in biological samples using DNA microarrays, we employed reference DNA to normalize variations in spot size and hybridization. This method was tested using nitrate reductase (nirS), naphthalene dioxygenase (nahA), and Escherichia coli O157 O-antigen biosynthesis genes as model genes and lambda DNA as the reference DNA. We observed a good linearity between the log signal ratio and log DNA concentration ratio at DNA concentrations above the method's detection limit, which was approximately 10 pg. This approach for designing quantitative microarrays and the inferred equation from this study provide a simple and convenient way to estimate the target gene concentration from the hybridization signal ratio.
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