ArticleApplied and environmental microbiology2002
Molecular cloning of XYL3 (D-xylulokinase) from Pichia stipitis and characterization of its physiological function.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Recent progress in engineering yeast producers of cellulosic ethanol.FEMS yeast research · 2025Review
- Engineering of xylose metabolic pathways in Rhodotorula toruloides for sustainable biomanufacturing.FEMS yeast research · 2025Review
- Exploring Yeast Diversity to Produce Lipid-Based Biofuels from Agro-Forestry and Industrial Organic Residues.Journal of fungi (Basel, Switzerland) · 2022Review
- Genotypic and phenotypic diversity among Komagataella species reveals a hidden pathway for xylose utilization.Microbial cell factories · 2022Review
- Construction of advanced producers of first- and second-generation ethanol in Saccharomyces cerevisiae and selected species of non-conventional yeasts (Scheffersomyces stipitis, Ogataea polymorpha).Journal of industrial microbiology & biotechnology · 2020Review
- Engineering microbial pathways for production of bio-based chemicals from lignocellulosic sugars: current status and perspectives.Biotechnology for biofuels · 2020Review
- PKA and HOG signaling contribute separable roles to anaerobic xylose fermentation in yeast engineered for biofuel production.PloS one · 2019Article
- Production of fuels and chemicals from xylose by engineered Saccharomyces cerevisiae: a review and perspective.Microbial cell factories · 2017Review
- Engineering of Saccharomyces cerevisiae to utilize xylan as a sole carbohydrate source by co-expression of an endoxylanase, xylosidase and a bacterial xylose isomerase.Journal of industrial microbiology & biotechnology · 2016Article
- Activating and Elucidating Metabolism of Complex Sugars in Yarrowia lipolytica.Applied and environmental microbiology · 2016Article
- Bench-scale bioethanol production from eucalyptus by high solid saccharification and glucose/xylose fermentation method.Bioprocess and biosystems engineering · 2014Article
- A constraint-based model of Scheffersomyces stipitis for improved ethanol production.Biotechnology for biofuels · 2012Article
- Xylitol production from DEO hydrolysate of corn stover by Pichia stipitis YS-30.Journal of industrial microbiology & biotechnology · 2011Article
- Identification of a xylulokinase catalyzing xylulose phosphorylation in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777.Journal of industrial microbiology & biotechnology · 2011Article
- Chemical and Synthetic Genetic Array Analysis Identifies Genes that Suppress Xylose Utilization and Fermentation in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2011Article
- Bulk segregant analysis by high-throughput sequencing reveals a novel xylose utilization gene from Saccharomyces cerevisiae.PLoS genetics · 2010Article
- Engineering for biofuels: exploiting innate microbial capacity or importing biosynthetic potential?Nature reviews. Microbiology · 2009Review
- Pichia stipitis genomics, transcriptomics, and gene clusters.FEMS yeast research · 2009Review
- DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways.Nucleic acids research · 2009Article
- Use of in vivo 13C nuclear magnetic resonance spectroscopy to elucidate L-arabinose metabolism in yeasts.Applied and environmental microbiology · 2008Article
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4 authors.
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Abstract
XYL3, which encodes a D-xylulokinase (EC 2.7.1.17), was isolated from Pichia stipitis CBS 6054 genomic DNA by using primers designed against conserved motifs. Disruption of XYL3 eliminated D-xylulokinase activity, but D-ribulokinase activity was still present. Southern analysis of P. stipitis genomic DNA with XYL3 as a probe confirmed the disruption and did not reveal additional related genes. Disruption of XYL3 stopped ethanol production from xylose, but the resulting mutant still assimilated xylose slowly and formed xylitol and arabinitol. These results indicate that XYL3 is critical for ethanol production from xylose but that P. stipitis has another pathway for xylose assimilation. Expression of XYL3 using its P. stipitis promoter increased Saccharomyces cerevisiae D-xylulose consumption threefold and enabled the transformants to produce ethanol from a mixture of xylose and xylulose, whereas the parental strain only accumulated xylitol. In vitro, D-xylulokinase activity in recombinant S. cerevisiae was sixfold higher with a multicopy than with a single-copy XYL3 plasmid, but ethanol production decreased with increased copy number. These results confirmed the function of XYL3 in S. cerevisiae.
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