ArticleApplied and environmental microbiology2002
Characterization of a chitinase gene from Stenotrophomonas maltophilia strain 34S1 and its involvement in biological control.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 1 of them a synthesis that pooled it.
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Who cites it
44 citing papers in PubMed, 1 synthesis or guideline pooled it, 166 citations in OpenAlex.
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- Plant Beneficial Bacteria and Their Potential Applications in Vertical Farming Systems.Plants (Basel, Switzerland) · 2023Review
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- Evaluation of Endospore-Forming Bacteria for Suppression of Postharvest Decay of Apple Fruit.Microorganisms · 2022Article
- Molecular analysis of genes involved in chitin degradation from the chitinolytic bacterium Bacillus velezensis.Antonie van Leeuwenhoek · 2022Article
- Antifungal Properties, Abiotic Stress Resistance, and Biocontrol Ability of Bacillus mojavensis PS17.Current microbiology · 2021Article
- Distinct Compartmentalization of Microbial Community and Potential Metabolic Function in the Fruiting Body ofJournal of fungi (Basel, Switzerland) · 2021Article
- Outer Membrane Vesicle-Mediated Codelivery of the Antifungal HSAF Metabolites and Lytic Polysaccharide Monooxygenase in the PredatoryACS chemical biology · 2021Article
- Biotechnological potential of bacteria from genera Bacillus Paraburkholderia and Pseudomonas to control seed fungal pathogens.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Comparative Genomics ofInternational journal of molecular sciences · 2020Article
- Development and Application of Low-Cost and Eco-Sustainable Bio-Stimulant Containing a New Plant Growth-Promoting StrainFrontiers in microbiology · 2020Article
- The Microbiome of Leonardo da Vinci's Drawings: A Bio-Archive of Their History.Frontiers in microbiology · 2020Article
- Purification, characterization and cloning of a chitinase from3 Biotech · 2020Article
- Isolation and Characterization of Antagonistic BacteriaBioMed research international · 2019Article
- Article
- Effect of fruiting body bacteria on the growth of Tricholoma matsutake and its related molds.PloS one · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A chitinase gene was cloned on a 2.8-kb DNA fragment from Stenotrophomonas maltophilia strain 34S1 by heterologous expression in Burkholderia cepacia. Sequence analysis of this fragment identified an open reading frame encoding a deduced protein of 700 amino acids. Removal of the signal peptide sequence resulted in a predicted protein that was 68 kDa in size. Analysis of the sequence indicated that the chitinase contained a catalytic domain belonging to family 18 of glycosyl hydrolases. Three putative binding domains, a chitin binding domain, a novel polycystic kidney disease (PKD) domain, and a fibronectin type III domain, were also identified within the sequence. Pairwise comparisons of each domain to the most closely related sequences found in database searches clearly demonstrated variation in gene sources and the species from which related sequences originated. A 51-kDa protein with chitinolytic activity was purified from culture filtrates of S. maltophilia strain 34S1 by hydrophobic interaction chromatography. Although the protein was significantly smaller than the size predicted from the sequence, the N-terminal sequence verified that the first 15 amino acids were identical to the deduced sequence of the mature protein encoded by chiA. Marker exchange mutagenesis of chiA resulted in mutant strain C5, which was devoid of chitinolytic activity and lacked the 51-kDa protein in culture filtrates. Strain C5 was also reduced in the ability to suppress summer patch disease on Kentucky bluegrass, supporting a role for the enzyme in the biocontrol activity of S. maltophilia.
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