ArticleBritish journal of cancer2002
Hypoxia-associated spontaneous pulmonary metastasis in human melanoma xenografts: involvement of microvascular hot spots induced in hypoxic foci by interleukin 8.
Article in British journal of cancer, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 76 citations in OpenAlex.
- Circulating proteins as potential biomarkers of sunitinib and interferon-α efficacy in treatment-naïve patients with metastatic renal cell carcinoma.Cancer chemotherapy and pharmacology · 2014Trial
- Lenalidomide modulates IL-8 and anti-prostate antibody levels in men with biochemically recurrent prostate cancer.The Prostate · 2012Trial
- Physiological elasticity and topography modulateiScience · 2026Article
- Emerging organoids and organoids-on-chip platforms for translational development of antibody‒drug conjugates and next-generation bioconjugates.Acta pharmaceutica Sinica. B · 2026Review
- From 2D to 3D and beyond: the evolution and impact of in vitro tumor models in cancer research.Nature methods · 2025Review
- Review
- ALDH1A1 overexpression in melanoma cells promotes tumor angiogenesis by activating the IL‑8/Notch signaling cascade.International journal of molecular medicine · 2022Article
- The Effect of Hypoxia on the Expression of CXC Chemokines and CXC Chemokine Receptors-A Review of Literature.International journal of molecular sciences · 2021Review
- Can anticancer chemotherapy promote the progression of brain metastases?Medical oncology (Northwood, London, England) · 2018Article
- The Effect of Sunitinib Treatment in Human Melanoma Xenografts: Associations with Angiogenic Profiles.Translational oncology · 2017Article
- Stimuli-induced organ-specific injury enhancement of organotropic metastasis in a spatiotemporal regulation.Pathology oncology research : POR · 2014Review
- Molecular mechanisms involved in tumor repopulation after radiotherapy.Translational cancer research · 2013Article
- Effect of culture at low oxygen tension on the expression of heat shock proteins in a panel of melanoma cell lines.PloS one · 2012Article
- Antiangiogenic therapy: impact on invasion, disease progression, and metastasis.Nature reviews. Clinical oncology · 2011Review
- Metastasis in melanoma xenografts is associated with tumor microvascular density rather than extent of hypoxia.Neoplasia (New York, N.Y.) · 2010Article
- Three-dimensional multispecies nonlinear tumor growth-II: Tumor invasion and angiogenesis.Journal of theoretical biology · 2010Article
- Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.Molecular & cellular proteomics : MCP · 2010Article
- Angiogenesis and melanoma.Cancers · 2010Article
- Nonlinear modelling of cancer: bridging the gap between cells and tumours.Nonlinearity · 2010Article
- Comparative expression pathway analysis of human and canine mammary tumors.BMC genomics · 2009Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to investigate whether tumour hypoxia and/or vascular hot spots promote the development of metastatic disease. The D-12 human melanoma xenograft line was used as a tumour model. Hypoxia and vascular hot spots were detected by immunohistochemistry using pimonidazole as a hypoxia marker and anti-CD31 antibody to visualize endothelial cells. Vascular hot spots were found to be induced in hypoxic foci, owing to hypoxia-induced up-regulation of angiogenesis stimulatory factors. This effect was mediated by interleukin 8 and possibly also by vascular endothelial growth factor. Interleukin 8 positive foci showed a high degree of co-localization with hypoxic foci, as revealed by immunohistochemistry. The incidence of spontaneous pulmonary metastases was associated with the density of hypoxic foci, the density of interleukin 8 positive foci and the density of vascular hot spots in the primary tumour. Treatment with neutralizing antibody against interleukin 8 and/or vascular endothelial growth factor resulted in hypoxia-induced necrosis rather than hypoxia-induced vascular hot spots and inhibited metastasis. Our study suggests a cause-effect relationship between hypoxia and metastasis in cancer and hence an elevated probability of metastatic disease in patients having primary tumours characterized by high densities of hypoxic foci and vascular hot spots.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.