ArticleProceedings of the National Academy of Sciences of the United States of America2002
Conserved regulation of the lymphocyte-specific expression of lck in the Fugu and mammals.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 57 citations in OpenAlex.
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- A novel cell-based binding assay system reconstituting interaction between SARS-CoV S protein and its cellular receptor.Journal of virological methods · 2005Article
- An enigmatic fourth runt domain gene in the fugu genome: ancestral gene loss versus accelerated evolution.BMC evolutionary biology · 2004Article
- Cloning, expression, and characterization of fugu CD4, the first ectothermic animal CD4.Immunogenetics · 2004Article
- Fugu ESTs: new resources for transcription analysis and genome annotation.Genome research · 2003Article
- Evolutionary conservation of regulatory elements in vertebrate Hox gene clusters.Genome research · 2003Article
- The human L-threonine 3-dehydrogenase gene is an expressed pseudogene.BMC genetics · 2002Article
- Sushi gets serious: the draft genome sequence of the pufferfish Fugu rubripes.Genome biology · 2002Review
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8 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The lck gene encodes a lymphocyte-specific protein-tyrosine kinase that is implicated in T cell maturation and signaling. In mammals, the transcription of the lck gene is regulated by two independent promoters, the proximal promoter, which is active in thymocytes, and the distal promoter, which dominates in mature T cells. In the human and mouse lck gene loci, the two promoter elements are separated by at least 40 kb and 10 kb, respectively. In this study, we have cloned and sequenced 60 kb from the pufferfish (Fugu rubripes) lck locus. The promoter region of the Fugu lck spans only 4.2 kb and contains a proximal and a distal promoter in the 2.3-kb region adjacent to the coding sequence. By generating transgenic mice, we have demonstrated that the compact promoter of the Fugu lck contains regulatory elements that direct expression to lymphoid organs of mice. We were able to localize the regulatory elements to a short region of 830 bp without losing specificity to cultured human T cell line. These results show that the basic mechanisms that mediate lymphocyte-specific expression are conserved between teleosts and mammals. The short promoter of the Fugu lck isolated by us offers a powerful tool for labeling T cells, targeting expression, and manipulating T cell activity in fishes as well as in mammals.
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