ArticleThe EMBO journal2002
Visualization of the ER-to-cytosol dislocation reaction of a type I membrane protein.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 85 citations in OpenAlex.
- Identification of two rate-limiting steps in the degradation of partially folded immunoglobulin light chains.Frontiers in cell and developmental biology · 2022Article
- Article
- BiP/GRP78 Mediates ERAD Targeting of Proteins Produced by Membrane-Bound Ribosomes Stalled at the STOP-Codon.Journal of molecular biology · 2019Article
- Regulation of pollen lipid body biogenesis by MAP kinases and downstream WRKY transcription factors in Arabidopsis.PLoS genetics · 2018Article
- Review
- Quality control: ER-associated degradation: protein quality control and beyond.The Journal of cell biology · 2014Review
- CD4 and BST-2/tetherin proteins retro-translocate from endoplasmic reticulum to cytosol as partially folded and multimeric molecules.The Journal of biological chemistry · 2014Article
- The mammalian endoplasmic reticulum-associated degradation system.Cold Spring Harbor perspectives in biology · 2013Review
- Roles of p97-associated deubiquitinases in protein quality control at the endoplasmic reticulum.Current protein & peptide science · 2012Review
- The delicate balance between secreted protein folding and endoplasmic reticulum-associated degradation in human physiology.Physiological reviews · 2012Review
- Protein disulfide isomerases contribute differentially to the endoplasmic reticulum-associated degradation of apolipoprotein B and other substrates.Molecular biology of the cell · 2012Article
- Protein quality control in the ER: balancing the ubiquitin checkbook.Trends in cell biology · 2012Review
- Live-cell imaging of ubiquitin-proteasome system function.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Ubiquitylation in ERAD: reversing to go forward?PLoS biology · 2011Article
- The signal peptide of the IgE receptor alpha-chain prevents surface expression of an immunoreceptor tyrosine-based activation motif-free receptor pool.The Journal of biological chemistry · 2010Article
- Article
- The TRC8 E3 ligase ubiquitinates MHC class I molecules before dislocation from the ER.The Journal of cell biology · 2009Article
- The first transmembrane region of the beta-chain stabilizes the tetrameric Fc epsilon RI complex.Molecular immunology · 2009Article
- A novel mode of translocation for cytolethal distending toxin.Biochimica et biophysica acta · 2009Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The human cytomegalovirus gene products US2 and US11 induce proteasomal degradation of MHC class I heavy chains. We have generated an enhanced green fluorescent protein-class I heavy chain (EGFP-HC) chimeric molecule to study its dislocation and degradation in US2- and US11-expressing cells. The EGFP-HC fusion is stable in control cells, but is degraded rapidly in US2- or US11-expressing cells. Proteasome inhibitors induce in a time-dependent manner the accumulation of EGFP-HC molecules in US2- and US11-expressing cells, as assessed biochemically and by cytofluorimetry of intact cells. Pulse-chase analysis and subcellular fractionation show that EGFP-HC proteins are dislocated from the endoplasmic reticulum and can be recovered as deglycosylated fluorescent intermediates in the cytosol. These results raise the possibility that dislocation of glycoproteins from the ER may not require their full unfolding.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.