ArticleBiophysical journal2002
Ca(2+)-induced movement of tropomyosin in skeletal muscle thin filaments observed by multi-site FRET.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 51 citations in OpenAlex.
- Intermolecular And Dynamic Investigation of The Mechanism of Action of Reldesemtiv on Fast Skeletal Muscle Troponin Complex Toward the Treatment of Impaired Muscle Function.The protein journal · 2023Article
- A Stochastic Multiscale Model of Cardiac Thin Filament Activation Using Brownian-Langevin Dynamics.Biophysical journal · 2019Article
- Tropomyosin dynamics during cardiac muscle contraction as governed by a multi-well energy landscape.Progress in biophysics and molecular biology · 2019Article
- Clinically Divergent Mutation Effects on the Structure and Function of the Human Cardiac Tropomyosin Overlap.Biochemistry · 2017Article
- Direct observation of tropomyosin binding to actin filaments.Cytoskeleton (Hoboken, N.J.) · 2015Article
- In situ time-resolved FRET reveals effects of sarcomere length on cardiac thin-filament activation.Biophysical journal · 2014Article
- Tropomyosin dynamics.Journal of muscle research and cell motility · 2014Review
- Length-dependent effects on cardiac contractile dynamics are different in cardiac muscle containing α- or β-myosin heavy chain.Archives of biochemistry and biophysics · 2013Article
- Structure and dynamics of the actin-based smooth muscle contractile and cytoskeletal apparatus.Journal of muscle research and cell motility · 2012Review
- Nitroxyl-mediated disulfide bond formation between cardiac myofilament cysteines enhances contractile function.Circulation research · 2012Article
- Article
- Interaction sites of tropomyosin in muscle thin filament as identified by site-directed spin-labeling.Biophysical journal · 2011Article
- Gene transfer, expression, and sarcomeric incorporation of a headless myosin molecule in cardiac myocytes: evidence for a reserve in myofilament motor function.American journal of physiology. Heart and circulatory physiology · 2011Article
- Switch action of troponin on muscle thin filament as revealed by spin labeling and pulsed EPR.The Journal of biological chemistry · 2010Article
- Large-scale models reveal the two-component mechanics of striated muscle.International journal of molecular sciences · 2008Article
- Fast pressure jumps can perturb calcium and magnesium binding to troponin C F29W.Biochemistry · 2008Article
- Tropomyosin dynamics in cardiac thin filaments: a multisite forster resonance energy transfer and anisotropy study.Biophysical journal · 2008Article
- Different effects of trifluoroethanol and glycerol on the stability of tropomyosin helices and the head-to-tail complex.Biophysical journal · 2007Article
- Differential interaction of cardiac, skeletal muscle, and yeast tropomyosins with fluorescent (pyrene235) yeast actin.Biophysical journal · 2006Article
- Thymosin beta4 induces a conformational change in actin monomers.Biophysical journal · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
To obtain information on Ca(2+)-induced tropomyosin (Tm) movement in Ca(2+)-regulated muscle thin filaments, frequency-domain fluorescence energy transfer data were collected between 5-(2-iodoacetyl-amino-ethyl-amino)naphthalene-1-sulfonic acid at Cys-190 of Tm and phalloidin-tetramethylrhodamine B isothiocyanate bound to F-actin. Two models were used to fit the experimental data: an atomic coordinate (AC) model coupled with a search algorithm that varies the position and orientation of Tm on F-actin, and a double Gaussian distance distribution (DD) model. The AC model showed that little or no change in transfer efficiency is to be expected between different sites on F-actin and Tm if Ca(2+) causes azimuthal movement of Tm of the magnitude suggested by structural data (C. Xu, R. Craig, L. Tobacman, R. Horowitz, and W. Lehman. 1999. Biophys. J. 77:985-992). However, Ca(2+) produced a small but significant change in our phase/modulation versus frequency data, showing that changes in lifetime decay can be detected even when a change of the steady-state transfer efficiency is very small. A change in Tm azimuthal position of 17 on the actin filament obtained with the AC model indicates that solution data are in reasonable agreement with EM image reconstruction data. In addition, the data indicate that Tm also appears to rotate about its axis, resulting in a rolling motion over the F-actin surface. The DD model showed that the distance from one of the two chains of Tm to F-actin was mainly affected, further verifying that Ca(2+) causes Tm to roll over the F-actin surface. The width of the distance distributions indicated that the position of Tm in absence and in presence of Ca(2+) is well defined with appreciable local flexibility.
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