ArticleBiophysical journal2002
Structure, composition, and peptide binding properties of detergent soluble bilayers and detergent resistant rafts.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed, 208 citations in OpenAlex.
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- Roles of Membrane Domains in Integrin-Mediated Cell Adhesion.International journal of molecular sciences · 2020Review
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- Dynamic pattern generation in cell membranes: Current insights into membrane organization.Biochimica et biophysica acta. Biomembranes · 2018Review
- Changes in Cholesterol Level Alter Integrin Sequestration in Raft-Mimicking Lipid Mixtures.Biophysical journal · 2018Article
- Proteomic Analysis of Plasmodesmata FromFrontiers in plant science · 2018Article
- Complex biomembrane mimetics on the sub-nanometer scale.Biophysical reviews · 2017Review
- Clinical concentrations of chemically diverse general anesthetics minimally affect lipid bilayer properties.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Three-Phase Coexistence in Lipid Membranes.Biophysical journal · 2017Article
- The Role of Signaling via Aqueous Pore Formation in Resistance Responses to Amphotericin B.Antimicrobial agents and chemotherapy · 2016Review
- Influenza M2 Transmembrane Domain Senses Membrane Heterogeneity and Enhances Membrane Curvature.Langmuir : the ACS journal of surfaces and colloids · 2016Article
- Determination of mosaicity in oriented stacks of lipid bilayers.Soft matter · 2016Article
- Cholesterol-Enriched Domain Formation Induced by Viral-Encoded, Membrane-Active Amphipathic Peptide.Biophysical journal · 2016Article
- A general mechanism for drug promiscuity: Studies with amiodarone and other antiarrhythmics.The Journal of general physiology · 2015Article
- A Parameterization of Cholesterol for Mixed Lipid Bilayer Simulation within the Amber Lipid14 Force Field.The journal of physical chemistry. B · 2015Article
- Elastic Membrane Deformations Govern Interleaflet Coupling of Lipid-Ordered Domains.Physical review letters · 2015Article
29 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Lipid bilayers composed of unsaturated phosphatidylcholine (PC), sphingomyelin (SM), and cholesterol are thought to contain microdomains that have similar detergent insolubility characteristics as rafts isolated from cell plasma membranes. We chemically characterized the fractions corresponding to detergent soluble membranes (DSMs) and detergent resistant membranes (DRMs) from 1:1:1 PC:SM:cholesterol, compared the binding properties of selected peptides to bilayers with the compositions of DSMs and DRMs, used differential scanning calorimetry to identify phase transitions, and determined the structure of DRMs with x-ray diffraction. Compared with the equimolar starting material, DRMs were enriched in both SM and cholesterol. Both transmembrane and interfacial peptides bound to a greater extent to DSM bilayers than to DRM bilayers, likely because of differences in the mechanical properties of the two bilayers. Thermograms from 1:1:1 PC:SM:cholesterol from 3 to 70 degrees C showed no evidence for a liquid-ordered to liquid-disordered phase transition. Over a wide range of osmotic stresses, each x-ray pattern from equimolar PC:SM:cholesterol or DRMs contained a broad wide-angle band at 4.5 A, indicating that the bilayers were in a liquid-crystalline phase, and several sharp low-angle reflections that indexed as orders of a single lamellar repeat period. Electron density profiles showed that the total bilayer thickness was 57 A for DRMs, which was approximately 5 A greater than that of 1:1:1 PC:SM:cholesterol and 10 A greater than the thickness of bilayers with the composition of DSMs. These x-ray data provide accurate values for the widths of raft and nonraft bilayers that should be important in understanding mechanisms of protein sorting by rafts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.