ArticleMolecular and cellular biology2002
RNA destabilization by the granulocyte colony-stimulating factor stem-loop destabilizing element involves a single stem-loop that promotes deadenylation.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Identification of new high affinity targets for Roquin based on structural conservation.Nucleic acids research · 2018Article
- Short-lived AUF1 p42-binding mRNAs of RANKL and BCL6 have two distinct instability elements each.PloS one · 2018Article
- SB203580 increases G-CSF production via a stem-loop destabilizing element in the 3' untranslated region in macrophages independently of its effect on p38 MAPK activity.Journal of biomedical science · 2016Article
- RNase-L control of cellular mRNAs: roles in biologic functions and mechanisms of substrate targeting.Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · 2014Review
- Post-transcriptional regulatory networks in immunity.Immunological reviews · 2013Review
- A Type II Arabinogalactan from Anoectochilus formosanus for G-CSF Production in Macrophages and Leukopenia Improvement in CT26-Bearing Mice Treated with 5-Fluorouracil.Evidence-based complementary and alternative medicine : eCAM · 2013Article
- New families of human regulatory RNA structures identified by comparative analysis of vertebrate genomes.Genome research · 2011Article
- mRNA degradation plays a significant role in the program of gene expression regulated by phosphatidylinositol 3-kinase signaling.Molecular and cellular biology · 2010Article
- Post-transcriptional regulons coordinate the initiation and resolution of inflammation.Nature reviews. Immunology · 2010Review
- Destabilization of interleukin-6 mRNA requires a putative RNA stem-loop structure, an AU-rich element, and the RNA-binding protein AUF1.Molecular and cellular biology · 2006Article
- Regulation of alpha-synuclein expression in alcohol-preferring and -non preferring rats.Journal of neurochemistry · 2006Article
- Review
- Identification of signaling components required for the prediction of cytokine release in RAW 264.7 macrophages.Genome biology · 2006Article
- Using RNA secondary structures to guide sequence motif finding towards single-stranded regions.Nucleic acids research · 2006Article
- Common variations in noncoding regions of the human natriuretic peptide receptor A gene have quantitative effects.Human genetics · 2003Article
- Proinflammatory mediators, stimulators of sensory neuron excitability via the expression of acid-sensing ion channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Granulocyte colony-stimulating factor (G-CSF) mRNA contains two distinct types of cis-acting mRNA destabilizing elements in the 3'-untranslated region. In addition to several copies of the AU-rich element the G-CSF mRNA also contains a destabilizing region that includes several predicted stem-loop structures. We report here that the destabilizing activity resides in a single stem-loop structure within this region. A consensus sequence for the active structure has been derived by site-directed mutagenesis, revealing that a three-base loop of sequence YAU and unpaired bases either side of the stem contribute to the activity. The helical nature of the stem is essential and the stem must be less than 11 bp in length, but the destabilizing activity is relatively insensitive to the sequence within the helix. The stem-loop increases the rate of mRNA deadenylation, most likely by enhancing the processivity of the deadenylation reaction. A protein that binds the stem-loop, but not an inactive mutant form, has been detected in cytoplasmic lysates.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.