ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2002
Chromosome sorting and PCR-based physical mapping in pea (Pisum sativum L.).
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 62 citations in OpenAlex.
- Genetic mapping identifies QTL and candidate genes including Mendel's A gene controlling high levels of partial resistance to Fusarium avenaceum in Pea.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- KNL1 and NDC80 represent new universal markers for the detection of functional centromeres in plants.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2024Article
- Assembly of the 81.6 Mb centromere of pea chromosome 6 elucidates the structure and evolution of metapolycentric chromosomes.PLoS genetics · 2023Article
- Flow Cytometric Analysis and Sorting of Plant Chromosomes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Unraveling the Genetic Basis of Key Agronomic Traits of Wrinkled Vining Pea (Frontiers in plant science · 2022Article
- Mapping QTL associated with partial resistance to Aphanomyces root rot in pea (Pisum sativum L.) using a 13.2 K SNP array and SSR markers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Chromosome analysis and sorting.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Review
- Identification of Quantitative Trait Loci Associated With Partial Resistance to Fusarium Root Rot and Wilt Caused byFrontiers in plant science · 2021Article
- Extraordinary Sequence Diversity and Promiscuity of Centromeric Satellites in the Legume Tribe Fabeae.Molecular biology and evolution · 2020Article
- A reference genome for pea provides insight into legume genome evolution.Nature genetics · 2019Article
- Mitotic Spindle Attachment to the Holocentric Chromosomes ofFrontiers in plant science · 2019Article
- Satellite DNA in Vicia faba is characterized by remarkable diversity in its sequence composition, association with centromeres, and replication timing.Scientific reports · 2018Article
- Integration of Genetic and Cytogenetic Maps and Identification of Sex Chromosome in Garden Asparagus (Frontiers in plant science · 2018Article
- Molecular Cytogenetics ofBioMed research international · 2018Article
- Epigenetic Histone Marks of Extended Meta-Polycentric Centromeres of Lathyrus and Pisum Chromosomes.Frontiers in plant science · 2016Article
- Centromeres Off the Hook: Massive Changes in Centromere Size and Structure Following Duplication of CenH3 Gene in Fabeae Species.Molecular biology and evolution · 2015Article
- Chromosomes in the flow to simplify genome analysis.Functional & integrative genomics · 2012Review
- Stretching the rules: monocentric chromosomes with multiple centromere domains.PLoS genetics · 2012Article
- Integration of genetic and physical maps of the chickpea (Cicer arietinum L.) genome using flow-sorted chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- Unlocking the barley genome by chromosomal and comparative genomics.The Plant cell · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three pea lines with reconstructed karyotypes were used for analysis and subsequent purification of individual chromosome types using flow cytometry and sorting. The lines JI 145, JI 146, and JI 148 possess defined chromosomal translocations allowing discrimination of three to four chromosome types from each line based on the different sizes of translocation chromosomes. Whereas only two chromosomes could be sorted from standard (wild-type) karyotype, a combined use of these lines allowed sorting of six out of the seven types of pea chromosomes. Chromosomes were identified and purity of flow-sorted fractions was assessed using fluorescence in-situ hybridization with a PisTR-B probe that was previously shown to give labelling patterns characteristic for each chromosome type. The fractions of flow-sorted chromosomes were of very high purity (> 95%) and proved to be suitable for detection of gene and marker sequences using PCR with specific primers. Three fractions containing chromosomes 27, 72 and a pool of all remaining chromosomes (1, 3, 4, 5, 6) flow-sorted from the line JI 148 were then used for PCR-based physical localization of genetic markers selected from linkage groups IV and VII. These experiments enabled assignment of the linkage groups IV and VII to chromosomes 4 and 7, respectively.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.