ArticleNucleic acids research2002
An improved helper phage system for efficient isolation of specific antibody molecules in phage display.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Diversification of Phage-Displayed Peptide Libraries with Noncanonical Amino Acid Mutagenesis and Chemical Modification.Chemical reviews · 2024Review
- Design and Construction of a Synthetic Nanobody Library: Testing Its Potential with a Single Selection Round Strategy.Molecules (Basel, Switzerland) · 2023Article
- Antibody Isolation from Human Synthetic Libraries of Single-Chain Antibodies and Analysis Using NGS.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A Novel Synthetic Antibody Library with Complementarity-Determining Region Diversities Designed for an Improved Amplification Profile.International journal of molecular sciences · 2022Article
- Exhaustive Search of the Receptor Ligands by the CyCLOPS (Cytometry Cell-Labeling Operable Phage Screening) Technique.International journal of molecular sciences · 2020Article
- Tailoring subtractive cell biopanning to identify diffuse gastric adenocarcinoma-associated antigens via human scFv antibodies.Immunology · 2020Article
- A Sortase A Programmable Phage Display Format for Improved Panning of Fab Antibody Libraries.Journal of molecular biology · 2018Article
- Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody.Iranian journal of basic medical sciences · 2018Article
- Substitution of Heavy Complementarity Determining Region 3 (CDR-H3) Residues Can Synergistically Enhance Functional Activity of Antibody and Its Binding Affinity to HER2 Antigen.Molecules and cells · 2016Article
- Single Chain Antibodies Against gp55 of Human Cytomegalovirus (HCMV) for Prophylaxis and Treatment of HCMV Infections.Jundishapur journal of microbiology · 2016Article
- An improved single-chain Fab platform for efficient display and recombinant expression.Journal of molecular biology · 2015Article
- Bacteriophage vehicles for phage display: biology, mechanism, and application.Current microbiology · 2014Review
- DeltaPhage--a novel helper phage for high-valence pIX phagemid display.Nucleic acids research · 2012Article
- High affinity, developability and functional size: the holy grail of combinatorial antibody library generation.Molecules (Basel, Switzerland) · 2011Review
- Selection of antibodies against a single rare cell present in a heterogeneous population using phage display.Nature protocols · 2011Article
- Progress in phage display: evolution of the technique and its application.Cellular and molecular life sciences : CMLS · 2010Review
- A compact phage display human scFv library for selection of antibodies to a wide variety of antigens.BMC biotechnology · 2009Article
- On the influence of vector design on antibody phage display.Journal of biotechnology · 2007Article
- Eliminating helper phage from phage display.Nucleic acids research · 2006Article
- Toward cocaine esterase therapeutics.Journal of the American Chemical Society · 2005Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phage display technology has been applied in many fields of biological and medical sciences to study molecular interactions and especially in the generation of monoclonal antibodies of human origin. However, extremely low display level of antibody molecules on the surface of phage is an intrinsic problem of a phagemid-based display system resulting in low success rate of isolating specific binding molecules. We show here that display of single-chain antibody fragment (scFv) generated with pIGT3 phagemid can be increased dramatically by using a genetically modified Ex-phage. Ex-phage has a mutant pIII gene that produces a functional wild-type pIII in suppressing Escherichia coli strains but does not make any pIII in non-suppressing E.coli strains. Packaging phagemids encoding antibody-pIII fusion in F+ non-suppressing E.coli strains with Ex-phage enhanced the display level of antibody fragments on the surfaces of recombinant phage particles resulting in an increase of antigen-binding reactivity >100-fold compared to packaging with M13KO7 helper phage. Thus, the Ex-phage and pIGT3 phagemid vector provides a system for the efficient enrichment of specific binding antibodies from a phage display library and, thereby, increases the chance of obtaining more diverse antibodies specific for target antigens.
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