ArticleNucleic acids research2002
Magnesium is required for specific DNA binding of the CREB B-ZIP domain.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 64 citations in OpenAlex.
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- GAGA zinc finger transcription factor searches chromatin by 1D-3D facilitated diffusion.Nature structural & molecular biology · 2025Article
- Multi-faceted regulation of CREB family transcription factors.Frontiers in molecular neuroscience · 2024Review
- Continuous Real-Time Detection of Serotonin Using an Aptamer-Based Electrochemical Biosensor.Biosensors · 2023Article
- Magnesium and Liver Metabolism Through the Lifespan.Advances in nutrition (Bethesda, Md.) · 2023Review
- Identification of Orbivirus Non-Structural Protein 5 (NS5), Its Role and Interaction with RNA/DNA in Infected Cells.International journal of molecular sciences · 2023Article
- Coupling of binding and differential subdomain folding of the intrinsically disordered transcription factor CREB.FEBS letters · 2023Article
- A mini-TGA protein modulates gene expression through heterogeneous association with transcription factors.Plant physiology · 2023Article
- SEMplMe: a tool for integrating DNA methylation effects in transcription factor binding affinity predictions.BMC bioinformatics · 2022Article
- C/EBPβ regulates the JAK/STAT signaling pathway in triple-negative breast cancer.FEBS open bio · 2021Article
- In-cell infrared difference spectroscopy of LOV photoreceptors reveals structural responses to light altered in living cells.The Journal of biological chemistry · 2020Article
- Divalent cations promote TALE DNA-binding specificity.Nucleic acids research · 2020Article
- Structural Basis for Graded Inhibition of CREB:DNA Interactions by Multisite Phosphorylation.Biochemistry · 2018Article
- Quantitative analyses of the equilibria among DNA complexes of a blue-light-regulated bZIP module, Photozipper.Biophysics and physicobiology · 2018Article
- A-ZIP53, a dominant negative reveals the molecular mechanism of heterodimerization between bZIP53, bZIP10 and bZIP25 involved in Arabidopsis seed maturation.Scientific reports · 2017Article
- Allosteric DNA nanoswitches for controlled release of a molecular cargo triggered by biological inputs.Chemical science · 2017Article
- 5-Methylcytosine (5mC) and 5-Hydroxymethylcytosine (5hmC) Enhance the DNA Binding of CREB1 to the C/EBP Half-Site Tetranucleotide GCAA.Biochemistry · 2016Article
- Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.Nucleic acids research · 2016Article
- Quantitative imaging of magnesium distribution at single-cell resolution in brain tumors and infiltrating tumor cells with secondary ion mass spectrometry (SIMS).Journal of neuro-oncology · 2016Article
Corrections and comments
- Erratum issuedGal Jozset [corrected to Gal Jozsef]
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have examined binding of the CREB B-ZIP protein domain to double-stranded DNA containing a consensus CRE sequence (5'-TGACGTCA-3'), the related PAR, C/EBP and AP-1 sequences and the unrelated SP1 sequence. DNA binding was assayed in the presence or absence of MgCl2 and/or KCl using two methods: circular dichroism (CD) spectroscopy and electrophoretic mobility shift assay (EMSA). The CD assay allows us to measure equilibrium binding in solution. Thermal denaturation in 150 mM KCl indicates that the CREB B-ZIP domain binds all the DNA sequences, with highest affinity for the CRE site, followed by the PAR (5'-TAACGTTA-3'), C/EBP (5'-TTGCGCAA-3') and AP-1 (5'-TGAGTCA-3') sites. The addition of 10 mM MgCl2 diminished DNA binding to the CRE and PAR DNA sequences and abolished binding to the C/EBP and AP-1 DNA sequences, resulting in more sequence-specific DNA binding. Using 'standard' EMSA conditions (0.25x TBE), CREB bound all the DNA sequences examined. The CREB-CRE complex had an apparent Kd of approximately 300 pM, PAR of approximately 1 nM, C/EBP and AP-1 of approximately 3 nM and SP1 of approximately 30 nM. The addition of 10 mM MgCl2 to the polyacrylamide gel dramatically altered sequence-specific DNA binding. CREB binding affinity for CRE DNA decreased 3-fold, but binding to the other DNA sequences decreased >1000-fold. In the EMSA, addition of 150 mM KCl to the gels had an effect similar to MgCl2. The magnesium concentration needed to prevent non-specific electrostatic interactions between CREB and DNA in solution is in the physiological range and thus changes in magnesium concentration may be a cellular signal that regulates gene expression.
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