ArticleNucleic acids research2002
SGS1 is a multicopy suppressor of srs2: functional overlap between DNA helicases.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 60 citations in OpenAlex.
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- Srs2 and Mus81-Mms4 Prevent Accumulation of Toxic Inter-Homolog Recombination Intermediates.PLoS genetics · 2016Article
- The HsRAD51B-HsRAD51C stabilizes the HsRAD51 nucleoprotein filament.DNA repair · 2013Article
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- Homologous recombination and its regulation.Nucleic acids research · 2012Review
- The Swi2-Snf2-like protein Uls1 is involved in replication stress response.Nucleic acids research · 2011Article
- Srs2 overexpression reveals a helicase-independent role at replication forks that requires diverse cell functions.DNA repair · 2011Article
- The SUMO isopeptidase Ulp2p is required to prevent recombination-induced chromosome segregation lethality following DNA replication stress.PLoS genetics · 2011Article
- Sgs1 and exo1 redundantly inhibit break-induced replication and de novo telomere addition at broken chromosome ends.PLoS genetics · 2010Article
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- Biochemistry of Meiotic Recombination: Formation, Processing, and Resolution of Recombination Intermediates.Genome dynamics and stability · 2008Article
- Novel pro- and anti-recombination activities of the Bloom's syndrome helicase.Genes & development · 2007Article
- Biochemistry of eukaryotic homologous recombination.Topics in current genetics · 2007Article
- Article
- Rad51-dependent DNA structures accumulate at damaged replication forks in sgs1 mutants defective in the yeast ortholog of BLM RecQ helicase.Genes & development · 2005Article
- Srs2 and RecQ homologs cooperate in mei-3-mediated homologous recombination repair of Neurospora crassa.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Sgs1 is a member of the RecQ family of DNA helicases, which have been implicated in genomic stability, cancer and ageing. Srs2 is another DNA helicase that shares several phenotypic features with Sgs1 and double sgs1srs2 mutants have a severe synthetic growth phenotype. This suggests that there may be functional overlap between these two DNA helicases. Consistent with this idea, we found the srs2Delta mutant to have a similar genotoxin sensitivity profile and replicative lifespan to the sgs1Delta mutant. In order to directly test if Sgs1 and Srs2 are functionally interchangeable, the ability of high-copy SGS1 and SRS2 plasmids to complement the srs2Delta and sgs1Delta mutants was assessed. We report here that SGS1 is a multicopy suppressor of the methyl methanesulphonate (MMS) and hydroxyurea sensitivity of the srs2Delta mutant, whereas SRS2 overexpression had no complementing ability in the sgs1Delta mutant. Domains of Sgs1 directly required for processing MMS-induced DNA damage, most notably the helicase domain, are also required for complementation of the srs2Delta mutant. Although SGS1 overexpression was unable to rescue the shortened mean replicative lifespan of the srs2Delta mutant, maximum lifespan was significantly increased by multicopy SGS1. We conclude that Sgs1 is able to partially compensate for the loss of Srs2.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.