ArticleJournal of virology2002
Alternative fates of keratinocytes transduced by human papillomavirus type 18 E7 during squamous differentiation.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- IDR-targeting compounds suppress HPV genome replication via disruption of phospho-BRD4 association with DNA damage response factors.Molecular cell · 2024Article
- Polyploid Giant Cancer Cells, a Hallmark of Oncoviruses and a New Therapeutic Challenge.Frontiers in oncology · 2020Review
- Role of Cdk1 in DNA damage-induced G1 checkpoint abrogation by the human papillomavirus E7 oncogene.Cell cycle (Georgetown, Tex.) · 2014Article
- Human papillomavirus infections: warts or cancer?Cold Spring Harbor perspectives in biology · 2013Review
- Evidences suggesting involvement of viruses in oral squamous cell carcinoma.Pathology research international · 2013Review
- Nonconserved lysine residues attenuate the biological function of the low-risk human papillomavirus E7 protein.Journal of virology · 2011Article
- Human papillomavirus (HPV) E7 induces prolonged G2 following S phase reentry in differentiated human keratinocytes.The Journal of biological chemistry · 2011Article
- Robust production and passaging of infectious HPV in squamous epithelium of primary human keratinocytes.Genes & development · 2009Article
- Conditionally activated E7 proteins of high-risk and low-risk human papillomaviruses induce S phase in postmitotic, differentiated human keratinocytes.Journal of virology · 2006Article
- The 3' region of human papillomavirus type 16 early mRNAs decrease expression.BMC infectious diseases · 2005Article
- Human papillomavirus type 16 E1circumflexE4 contributes to multiple facets of the papillomavirus life cycle.Journal of virology · 2005Article
- Cooperation between different forms of the human papillomavirus type 1 E4 protein to block cell cycle progression and cellular DNA synthesis.Journal of virology · 2004Article
- Global effects of human papillomavirus type 18 E6/E7 in an organotypic keratinocyte culture system.Journal of virology · 2004Article
- Induction of tetrasomy by human papillomavirus type 16 E7 protein is independent of pRb binding and disruption of differentiation.British journal of cancer · 2004Article
- HPV E7 expression in skeletal muscle cells distinguishes initiation of the postmitotic state from its maintenance.Oncogene · 2003Article
- Activation of adenovirus early promoters and lytic phase in differentiated strata of organotypic cultures of human keratinocytes.Journal of virology · 2003Article
- Inactivation of both the retinoblastoma tumor suppressor and p21 by the human papillomavirus type 16 E7 oncoprotein is necessary to inhibit cell cycle arrest in human epithelial cells.Journal of virology · 2002Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The human papillomavirus type 18 (HPV-18) E7 protein promotes S-phase reentry in postmitotic, differentiated keratinocytes in squamous epithelium to facilitate vegetative viral DNA amplification. To examine the nature and fate of the differentiated cells that reenter S phase, organotypic cultures of primary human keratinocytes transduced with HPV-18 E7 were pulse-chase-pulse-labeled with (3)H-thymidine ((3)H-TdR) and bromodeoxyuridine (BrdU). The kinetics of the appearance of doubly labeled suprabasal cells demonstrate that E7 expression did not promote prolonged S phase. Rather, there was a considerable lag before a small percentage of the cells reentered another round of S phase. Fluorescence in situ hybridization analysis, indeed, revealed a small fraction of the cells with more than 4n chromosomes in the differentiated strata. Differentiated cells positive for (3)H-TdR, BrdU, or both often had enlarged nuclei or were binucleated. These results suggest that S phase is not followed by cell division, although nuclear division may occur. Interestingly, a significant fraction of differentiated cells that entered S phase subsequently accumulated p27kip1 protein with a kinetics preceding the accumulation of cyclin E. We conclude that E7-transduced, differentiated keratinocytes that enter S phase have two alternative fates: (i) a low percentage of cells undergoes endoreduplication, achieving higher than 4n ploidy, and (ii) a high percentage of cells accumulates the p27kip1, cyclin E, and p21cip1 proteins, resulting in arrest and preventing further S-phase reentry.
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