ArticleGenetics2002
Assembly of two transgenes in an artificial chromatin domain gives highly coordinated expression in tobacco.
Article in Genetics, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 25 citations in OpenAlex.
- Unveiling the Impact of the Genomic Architecture on the Evolution of Vertebrate microRNAs.Frontiers in genetics · 2017Review
- Successive silencing of tandem reporter genes in potato (Solanum tuberosum) over 5 years of vegetative propagation.Annals of botany · 2010Article
- Modulation of chromatin boundary activities by nucleosome-remodeling activities in Drosophila melanogaster.Molecular and cellular biology · 2010Article
- Comparative analysis of divergent and convergent gene pairs and their expression patterns in rice, Arabidopsis, and populus.Plant physiology · 2008Article
- Local coexpression domains in the genome of rice show no microsynteny with Arabidopsis domains.Plant molecular biology · 2007Article
- Local coexpression domains of two to four genes in the genome of Arabidopsis.Plant physiology · 2005Article
- The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing.The Plant cell · 2003Article
- Analysis of trans-silencing interactions using transcriptional silencers of varying strength and targets with and without flanking nuclear matrix attachment regions.Transgenic research · 2003Article
- Epigenetic interactions between Arabidopsis transgenes: characterization in light of transgene integration sites.Plant molecular biology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chromatin loop model predicts that genes within the same chromatin domain exhibit coordinated regulation. We here present the first direct experimental support for this model in plants. Two reporter genes, the E. coli beta-glucuronidase gene and the firefly luciferase gene, driven by different promoters, were placed between copies of the chicken lysozyme A element, a member of the matrix-associated region (MAR) group of chromatin boundary elements, and introduced in tobacco (Nicotiana tabacum). In plants carrying A elements, quantitative enzyme activities and mRNA levels of both genes show high correlations compared to control plants. The A element thus creates an artificial chromatin domain that yields coordinated expression. Surprisingly, enzyme activities correlated poorly with their respective mRNA levels. We hypothesize that this indicates the occurrence of "error pipelines" in data generation: systematic errors of a given analytical method will point in the same direction and cancel out in correlation analysis, resulting in better correlations. In combining different methods of analysis, however, such errors do not cancel out and as a result relevant correlations can be masked. Such error pipelines will have to be taken into account when different types of (e.g., whole-genome) data sets are combined in quantitative analyses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.