ArticleGenetics2002
Identification of circadian-clock-regulated enhancers and genes of Drosophila melanogaster by transposon mobilization and luciferase reporting of cyclical gene expression.
Article in Genetics, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 37 citations in OpenAlex.
- Article
- Quasimodo mediates daily and acute light effects on Drosophila clock neuron excitability.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Gene profiling the response to repeated cocaine self-administration in dorsal striatum: a focus on circadian genes.Brain research · 2008Article
- A functional genomics strategy reveals clockwork orange as a transcriptional regulator in the Drosophila circadian clock.Genes & development · 2007Article
- The novel Drosophila tim(blind) mutation affects behavioral rhythms but not periodic eclosion.Genetics · 2005Article
- Pattern of distribution and cycling of SLOB, Slowpoke channel binding protein, in Drosophila.BMC neuroscience · 2004Article
- Enhancer trapping reveals widespread circadian clock transcriptional control in Arabidopsis.Plant physiology · 2003Article
- Genome-wide expression analysis in Drosophila reveals genes controlling circadian behavior.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Advanced analysis of a cryptochrome mutation's effects on the robustness and phase of molecular cycles in isolated peripheral tissues of Drosophila.BMC neuroscience · 2002Article
- Loss of circadian clock function decreases reproductive fitness in males of Drosophila melanogaster.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
A new way was developed to isolate rhythmically expressed genes in Drosophila by modifying the classic enhancer-trap method. We constructed a P element containing sequences that encode firefly luciferase as a reporter for oscillating gene expression in live flies. After generation of 1176 autosomal insertion lines, bioluminescence screening revealed rhythmic reporter-gene activity in 6% of these strains. Rhythmically fluctuating reporter levels were shown to be altered by clock mutations in genes that specify various circadian transcription factors or repressors. Intriguingly, rhythmic luminescence in certain lines was affected by only a subset of the pacemaker mutations. By isolating genes near 13 of the transposon insertions and determining their temporal mRNA expression pattern, we found that four of the loci adjacent to the trapped enhancers are rhythmically expressed. Therefore, this approach is suitable for identifying genetic loci regulated by the circadian clock. One transposon insert caused a mutation in the rhythmically expressed gene numb. This novel numb allele, as well as previously described ones, was shown to affect the fly's rhythm of locomotor activity. In addition to its known role in cell fate determination, this gene and the phosphotyrosine-binding protein it encodes are likely to function in the circadian system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.