ArticleGenetics2002
Unexpected stability of mariner transgenes in Drosophila.
Article in Genetics, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Article
- Mariner transposons are sailing in the genome of the blood-sucking bug Rhodnius prolixus.BMC genomics · 2015Article
- Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon.Nature methods · 2014Article
- A rice Stowaway MITE for gene transfer in yeast.PloS one · 2013Article
- A new powerful method for site-specific transgene stabilization based on chromosomal double-strand break repair.PloS one · 2011Article
- Delivering the goods: viral and non-viral gene therapy systems and the inherent limits on cargo DNA and internal sequences.Genetica · 2010Review
- Article
- Review
- Physical properties of DNA components affecting the transposition efficiency of the mariner Mos1 element.Molecular genetics and genomics : MGG · 2009Article
- Post-integration behavior of a Minos transposon in the malaria mosquito Anopheles stephensi.Molecular genetics and genomics : MGG · 2007Article
- Post-integration stability of piggyBac in Aedes aegypti.Insect biochemistry and molecular biology · 2007Article
- Technology transfer from worms and flies to vertebrates: transposition-based genome manipulations and their future perspectives.Genome biology · 2007Review
- Characterization of Mos1-mediated mutagenesis in Caenorhabditis elegans: a method for the rapid identification of mutated genes.Genetics · 2005Article
- An active transposable element, Herves, from the African malaria mosquito Anopheles gambiae.Genetics · 2005Article
- Post-integration stabilization of a transposon vector by terminal sequence deletion in Drosophila melanogaster.Nature biotechnology · 2004Article
- Analyses of cis -acting elements that affect the transposition of Mos1 mariner transposons in vivo.Molecular genetics and genomics : MGG · 2004Article
- Assessing fitness costs for transgenic Aedes aegypti expressing the GFP marker and transposase genes.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- piggyBac-based insertional mutagenesis and enhancer detection as a tool for functional insect genomics.Genetics · 2003Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
A number of mariner transformation vectors based on the mauritiana subfamily of transposable elements were introduced into the genome of Drosophila melanogaster and examined for their ability to be mobilized by the mariner transposase. Simple insertion vectors were constructed from single mariner elements into which exogenous DNA ranging in size from 1.3 to 4.5 kb had been inserted; composite vectors were constructed with partial or complete duplications of mariner flanking the exogenous DNA. All of the simple insertion vectors showed levels of somatic and germline excision that were at least 100-fold lower than the baseline level of uninterrupted mariner elements. Although composite vectors with inverted duplications were unable to be mobilized at detectable frequencies, vectors with large direct duplications of mariner could be mobilized. A vector consisting of two virtually complete elements flanking exogenous DNA yielded a frequency of somatic eye-color mosaicism of approximately 10% and a frequency of germline excision of 0.04%. These values are far smaller than those observed for uninterrupted elements. The results imply that efficient mobilization of mariner in vivo requires the presence and proper spacing of sequences internal to the element as well as the inverted repeats.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.