ArticleGenetics2002
Genetic control of extracellular protease synthesis in the yeast Yarrowia lipolytica.
Article in Genetics, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Yarrowia lipolytica and Komagataella phaffii (Pichia pastoris) as Hosts for Heterologous Expression of Lipases.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Engineering Saccharomyces cerevisiae secretory system for recombinant protein production: strategies from signal peptides to protease control.Archives of microbiology · 2026Review
- Characterization of alkaline protease enzyme produced from marine yeast Candida orthopsilosis AKB-1 and its applications.Folia microbiologica · 2025Article
- Insights into the efficient degradation mechanism of extracellular proteases mediated byFrontiers in microbiology · 2024Article
- Evaluation of the Potential of Lipid-ExtractedMarine drugs · 2022Article
- A Cumulative Effect by Multiple-Gene Knockout Strategy Leads to a Significant Increase in the Production of Sophorolipids inFrontiers in bioengineering and biotechnology · 2022Article
- Article
- Genomic analysis of novel Yarrowia-like yeast symbionts associated with the carrion-feeding burying beetle Nicrophorus vespilloides.BMC genomics · 2021Article
- pH Changes Have a Profound Effect on Gene Expression, Hydrolytic Enzyme Production, and Dimorphism inFrontiers in microbiology · 2021Article
- Identification and characterization of a protein Bro1 essential for sophorolipids synthesis in Starmerella bombicola.Journal of industrial microbiology & biotechnology · 2020Article
- Genetic Analysis of Four Sexual Differentiation Process Proteins (isp4/SDPs) inFrontiers in microbiology · 2019Article
- Article
- pH signaling in human fungal pathogens: a new target for antifungal strategies.Eukaryotic cell · 2014Review
- New insights into sulfur metabolism in yeasts as revealed by studies of Yarrowia lipolytica.Applied and environmental microbiology · 2013Article
- Reconstruction and in silico analysis of metabolic network for an oleaginous yeast, Yarrowia lipolytica.PloS one · 2012Article
- PalC, one of two Bro1 domain proteins in the fungal pH signalling pathway, localizes to cortical structures and binds Vps32.Traffic (Copenhagen, Denmark) · 2007Article
- The ambient pH response Rim pathway in Yarrowia lipolytica: identification of YlRIM9 and characterization of its role in dimorphism.Current microbiology · 2006Article
- Deletions of endocytic components VPS28 and VPS32 affect growth at alkaline pH and virulence through both RIM101-dependent and RIM101-independent pathways in Candida albicans.Infection and immunity · 2005Article
- Constitutive activation of the pH-responsive Rim101 pathway in yeast mutants defective in late steps of the MVB/ESCRT pathway.Molecular and cellular biology · 2005Article
- Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Depending on the pH of the growth medium, the yeast Yarrowia lipolytica secretes an acidic protease or an alkaline protease, the synthesis of which is also controlled by carbon, nitrogen, and sulfur availability, as well as by the presence of extracellular proteins. Previous results have indicated that the alkaline protease response to pH was dependent on YlRim101p, YlRim8p/YlPalF, and YlRim21p/YlPalH, three components of a conserved pH signaling pathway initially described in Aspergillus nidulans. To identify other partners of this response pathway, as well as pH-independent regulators of proteases, we searched for mutants that affect the expression of either or both acidic and alkaline proteases, using a YlmTn1-transposed genomic library. Four mutations affected only alkaline protease expression and identified the homolog of Saccharomyces cerevisiae SIN3. Eighty-nine mutations affected the expression of both proteases and identified 10 genes. Five of them define a conserved Rim pathway, which acts, as in other ascomycetes, by activating alkaline genes and repressing acidic genes at alkaline pH. Our results further suggest that in Y. lipolytica this pathway is active at acidic pH and is required for the expression of the acidic AXP1 gene. The five other genes are homologous to S. cerevisiae OPT1, SSY5, VPS28, NUP85, and MED4. YlOPT1 and YlSSY5 are not involved in pH sensing but define at least a second protease regulatory pathway.
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