ArticleBMC cell biology2002
Quantitation of the distribution and flux of myosin-II during cytokinesis.
Article in BMC cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 84 citations in OpenAlex.
- A continuum model of mechanosensation based on contractility kit assembly.Biophysical journal · 2025Article
- Mechanical power is maximized during contractile ring-like formation in a biomimetic dividing cell model.Nature communications · 2024Article
- Expanding ring-shaped cleavage furrows in multinucleate cells.Molecular biology of the cell · 2023Article
- Mechanics of stabilized intercellular bridges.Biophysical journal · 2022Article
- Patterning of the cell cortex and the localization of cleavage furrows in multi-nucleate cells.Journal of cell science · 2022Article
- Seeing is believing: tools to study the role of Rho GTPases during cytokinesis.Small GTPases · 2022Review
- Actomyosin Contraction Induces In-Bulk Motility of Cells and Droplets.Biophysical journal · 2020Article
- Article
- Tensile force-induced cytoskeletal remodeling: Mechanics before chemistry.PLoS computational biology · 2020Article
- Targeting Mechanoresponsive Proteins in Pancreatic Cancer: 4-Hydroxyacetophenone Blocks Dissemination and Invasion by Activating MYH14.Cancer research · 2019Article
- Molecular Mechanism of Cytokinesis.Annual review of biochemistry · 2019Review
- Myosin IIB assembly state determines its mechanosensitive dynamics.The Journal of cell biology · 2019Article
- 14-3-3 proteins tune non-muscle myosin II assembly.The Journal of biological chemistry · 2018Article
- Myosin efflux promotes cell elongation to coordinate chromosome segregation with cell cleavage.Nature communications · 2017Article
- Article
- A Combination of Actin Treadmilling and Cross-Linking Drives Contraction of Random Actomyosin Arrays.Biophysical journal · 2015Article
- Cell shape regulation through mechanosensory feedback control.Journal of the Royal Society, Interface · 2015Article
- Pharmacological activation of myosin II paralogs to correct cell mechanics defects.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The value of mechanistic biophysical information for systems-level understanding of complex biological processes such as cytokinesis.Biophysical journal · 2014Review
- Supervillin binding to myosin II and synergism with anillin are required for cytokinesis.Molecular biology of the cell · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundDuring cytokinesis, the cell's equator contracts against the cell's global stiffness. Identifying the biochemical basis for these mechanical parameters is essential for understanding how cells divide. To achieve this goal, the distribution and flux of the cell division machinery must be quantified. Here we report the first quantitative analysis of the distribution and flux of myosin-II, an essential element of the contractile ring.
resultsThe fluxes of myosin-II in the furrow cortex, the polar cortex, and the cytoplasm were examined using ratio imaging of GFP fusion proteins expressed in Dictyostelium. The peak concentration of GFP-myosin-II in the furrow cortex is 1.8-fold higher than in the polar cortex and 2.0-fold higher than in the cytoplasm. The myosin-II in the furrow cortex, however, represents only 10% of the total cellular myosin-II. An estimate of the minimal amount of this motor needed to produce the required force for cell cleavage fits well with this 10% value. The cell may, therefore, regulate the amount of myosin-II sent to the furrow cortex in accordance with the amount needed there. Quantitation of the distribution and flux of a mutant myosin-II that is defective in phosphorylation-dependent thick filament disassembly confirms that heavy chain phosphorylation regulates normal recruitment to the furrow cortex.
conclusionThe analysis indicates that myosin-II flux through the cleavage furrow cortex is regulated by thick filament phosphorylation. Further, the amount of myosin-II observed in the furrow cortex is in close agreement with the amount predicted to be required from a simple theoretical analysis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.