ArticleJournal of structural biology2001
Polymer-mediated compaction and internal dynamics of isolated Escherichia coli nucleoids.
Article in Journal of structural biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
53 citing papers in PubMed, 142 citations in OpenAlex.
- Review
- Structural Studies on the M. tuberculosis Nucleoid-associated-Protein, NapA, Indicates DNA Bridging Mechanism.Journal of molecular biology · 2025Article
- Bacterial chromosome conformation and cell-free gene expression in synthetic 2D compartments.Nature communications · 2025Article
- The role of active mRNA-ribosome dynamics and closing constriction in daughter chromosome separation inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- A microfluidic platform for extraction and analysis of bacterial genomic DNA.Lab on a chip · 2025Article
- Differentiating the roles of proteins and polysomes in nucleoid size homeostasis in Escherichia coli.Biophysical journal · 2024Article
- Compaction and Segregation of DNA inLife (Basel, Switzerland) · 2024Review
- Bacterial nucleoid is a riddle wrapped in a mystery inside an enigma.Journal of bacteriology · 2024Review
- Macromolecular Crowding, Phase Separation, and Homeostasis in the Orchestration of Bacterial Cellular Functions.Chemical reviews · 2024Review
- The Bacterial Nucleoid: From Electron Microscopy to Polymer Physics-A Personal Recollection.Life (Basel, Switzerland) · 2023Review
- DNA supercoiling in bacteria: state of play and challenges from a viewpoint of physics based modeling.Frontiers in microbiology · 2023Review
- Organization of the bacterial nucleoid by DNA-bridging proteins and globular crowders.Frontiers in microbiology · 2023Article
- Extracting and characterizing protein-free megabase-pair DNA forCell reports methods · 2022Article
- Review
- Review
- Steric interactions and out-of-equilibrium processes control the internal organization of bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Temporal compartmentalization of viral infection in bacterial cells.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Engineering spatiotemporal organization and dynamics in synthetic cells.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2021Review
- Self-assembly of multi-component mitochondrial nucleoids via phase separation.The EMBO journal · 2021Article
- Genome-in-a-Box: Building a Chromosome from the Bottom Up.ACS nano · 2021Review
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3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleoids of Escherichia coli were isolated by osmotic shock under conditions of low salt in the absence of added polyamines or Mg(2+). As determined by fluorescence microscopy, the isolated nucleoids in 0.2 M NaCl are expanded structures with an estimated volume of about 27 microm(3) according to a procedure based on a 50% threshold for the fluorescence intensity. The nucleoid volume is measured as a function of the concentration of added polyethylene glycol. The collapse is a continuous process, so that a coil-globule transition is not witnessed. The Helmholtz free energy of the nucleoids is determined via the depletion interaction between the DNA helix and the polyethylene glycol chains. The resulting compaction relation is discussed in terms of the current theory of branched DNA supercoils and it is concluded that the in vitro nucleoid is crosslinked in a physical sense. Despite the congested and crosslinked state of the nucleoid, the relaxation rate of its superhelical segments, as monitored by dynamic light scattering, turns out to be purely diffusional. At small scales, the nucleoid behaves as a fluid.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.