ArticleOncogene2002
PTEN regulates tumor cell adhesion of colon carcinoma cells under dynamic conditions of fluid flow.
Article in Oncogene, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- Age-associated changes in innate and adaptive immunity: role of the gut microbiota.Frontiers in immunology · 2024Review
- Fermentation Extract of Naringenin Increases the Expression of Estrogenic Receptor β and Modulates Genes Related to the p53 Signalling Pathway, miR-200c and miR-141 in Human Colon Cancer Cells Exposed to BPA.Molecules (Basel, Switzerland) · 2022Article
- The Role of PTEN in Epithelial-Mesenchymal Transition.Cancers · 2022Review
- [Effect of downregulation of phosphatase and tensin homolog gene expression on p130crk- related substrate protein and paxillin signal transduction in activated hepatic stellate cells in vitro].Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2019Article
- AKT1Molecular cancer research : MCR · 2019Article
- Melanoma central nervous system metastases: An update to approaches, challenges, and opportunities.Pigment cell & melanoma research · 2019Article
- Resveratrol inhibits Erk1/2-mediated adhesion of cancer cells via activating PP2A-PTEN signaling network.Journal of cellular physiology · 2019Article
- Endogenous Control Mechanisms of FAK and PYK2 and Their Relevance to Cancer Development.Cancers · 2018Review
- Clinical implications of PTEN and VEGF expression status, as well as microvessel density in esophageal squamous cell carcinoma.Oncology letters · 2015Article
- Phosphatase and Tensin Homolog (PTEN) Represses Colon Cancer Progression through Inhibiting Paxillin Transcription via PI3K/AKT/NF-κB Pathway.The Journal of biological chemistry · 2015Article
- Focus on PTEN Regulation.Frontiers in oncology · 2015Review
- PTEN function: the long and the short of it.Trends in biochemical sciences · 2014Review
- Akt directly regulates focal adhesion kinase through association and serine phosphorylation: implication for pressure-induced colon cancer metastasis.American journal of physiology. Cell physiology · 2011Article
- The PTEN phosphatase controls intestinal epithelial cell polarity and barrier function: role in colorectal cancer progression.PloS one · 2010Article
- In vivo tumor cell adhesion in the pulmonary microvasculature is exclusively mediated by tumor cell--endothelial cell interaction.BMC cancer · 2010Article
- Metastatic tumor cell arrest in the liver-lumen occlusion and specific adhesion are not exclusive.International journal of colorectal disease · 2009Article
- PCR-SSCP-DNA sequencing method in detecting PTEN gene mutation and its significance in human gastric cancer.World journal of gastroenterology · 2008Article
- NHERFs, NEP, MAGUKs, and more: interactions that regulate PTEN.Journal of cellular biochemistry · 2007Review
- A novel model to identify interaction partners of the PTEN tumor suppressor gene in human bladder cancer.Biochemical and biophysical research communications · 2007Article
- Organ-specific metastatic tumor cell adhesion and extravasation of colon carcinoma cells with different metastatic potential.The American journal of pathology · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The regulation of integrin-mediated cell adhesion and its stabilization involves different phosphorylation and dephosphorylation events. Focal adhesion kinase (FAK) has been recently found to be a substrate of the dual-specific phosphatase PTEN in glioma cells, where it appears to be involved in regulation of cell spreading and migration as part of focal adhesions. We have investigated the role of PTEN in cell adhesion of HT-29 human colon carcinoma cells under static and hydrodynamic conditions of fluid flow. PTEN coprecipitated with FAK and paxillin dependent on the formation of adhesions to collagens. This corresponded with an adhesion-dependent increase in Tyr-phosphatase activity of PTEN. Using preparations of native FAK and PTEN from HT-29 cells in a specific Tyr-phosphatase assay FAK was identified as substrate for this dephosphorylation. If expression of PTEN was reduced using antisense oligonucleotides cell adhesion under dynamic conditions of laminar flow, but not under static conditions was significantly increased. In addition, cell spreading was increased in cells with reduced PTEN expression. We conclude that PTEN appears to be involved in the regulation of integrin-mediated adhesion through dephosphorylation of FAK. This phosphatase might play a role as a negative regulator for the formation of stable HT-29 cell adhesion to extracellular matrix.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.