ArticleMolecular and cellular biochemistry2001
Identification and characterization of muscarinic acetylcholine receptor subtypes expressed in human skin melanocytes.
Article in Molecular and cellular biochemistry, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- VLA-4 integrin modulates cellular responses to sorafenib in metastasis-related processes of B16F10 melanoma cells.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Weight Gain With Advancing Age Is Controlled by the Muscarinic Acetylcholine Receptor M4 in Male Mice.Endocrinology · 2025Article
- A Comprehensive Review of Mammalian Pigmentation: Paving the Way for Innovative Hair Colour-Changing Cosmetics.Biology · 2023Review
- Endogenous DOPA inhibits melanoma through suppression of CHRM1 signaling.Science advances · 2022Article
- β-Lactam TRPM8 Antagonist RGM8-51 Displays Antinociceptive Activity in Different Animal Models.International journal of molecular sciences · 2022Article
- Role of Amine Neurotransmitters and Their Receptors in Skin Pigmentation: Therapeutic Implication.International journal of molecular sciences · 2021Review
- Multiple Roles for Cholinergic Signaling from the Perspective of Stem Cell Function.International journal of molecular sciences · 2021Review
- Review
- Ca2+ transients in melanocyte dendrites and dendritic spine-like structures evoked by cell-to-cell signaling.The Journal of cell biology · 2020Article
- Microphthalmia-associated transcription factor up-regulates acetylcholinesterase expression during melanogenesis of murine melanoma cells.The Journal of biological chemistry · 2018Article
- Cholinergic Machinery as Relevant Target in Acute Lymphoblastic T Leukemia.Frontiers in pharmacology · 2016Article
- The melanocyte photosensory system in the human skin.SpringerPlus · 2013Article
- Article
- Sensing the environment: regulation of local and global homeostasis by the skin's neuroendocrine system.Advances in anatomy, embryology, and cell biology · 2012Review
- Muscarinic receptor agonists and antagonists: effects on cancer.Handbook of experimental pharmacology · 2012Review
- Vertebrate melanophores as potential model for drug discovery and development: a review.Cellular & molecular biology letters · 2011Review
- Acetylcholine beyond neurons: the non-neuronal cholinergic system in humans.British journal of pharmacology · 2008Review
- The M4 muscarinic acetylcholine receptor plays a key role in the control of murine hair follicle cycling and pigmentation.Life sciences · 2007Article
- [The extraneuronal cholinergic system of the skin. Basic facts and clinical relevance].Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete · 2004Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study was designed to identify and characterize muscarinic acetylcholine receptors in normal human melanocytes. We used subtype-specific oligonucleotide primers to localize the five genetically defined mAChR mRNAs (ml through m5) by reverse transcription-polymerase chain reaction. These experiments showed that all five mAChR subtype mRNAs are expressed in melanocytes. The PCR products were verified by restriction analysis and Southern blotting. Receptors were visualized in cultures of normal human melanocytes and specimens of normal human skin by subtype-specific rabbit anti-receptor polyclonal antibodies. Radioligand binding assays with the lipophilic drug [3H]quinuclidinyl benzilate demonstrated approximately 9,000 high affinity binding sites/cell. Micromolar concentrations of muscarine or carbachol transiently increased intracellular Ca2+, which could be attenuated by atropine, demonstrating coupling of the receptors to mobilization of intracellular free Ca2+. Lower concentrations of muscarine induced spontaneous repetitive spike-like increases of intracellular Ca2+ which is characteristic for the activation of muscarinic receptors. These results indicate that normal human skin melanocytes express the ml, m2, m3, m4, and m5 subtypes of classic muscarinic acetylcholine receptors on their cell membrane and that these receptors regulate the concentration of intracellular free Ca2+, which may play an important physiologic role in melanocyte behavior and skin pigmentation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.