ArticleProceedings of the National Academy of Sciences of the United States of America2002
Homologous recombination at the border: insertion-deletions and the trapping of foreign DNA in Streptococcus pneumoniae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 98 citations in OpenAlex.
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- Harnessing CRISPR-Cas9 for Genome Editing in Streptococcus pneumoniae D39V.Applied and environmental microbiology · 2021Article
- Natural Horizontal Gene Transfer of Antimicrobial Resistance Genes inFrontiers in microbiology · 2021Article
- The Best of All Worlds:Microorganisms · 2019Review
- Recombination of the Phase-VariableJournal of bacteriology · 2019Article
- Genomics and Genetics ofMicrobiology spectrum · 2019Review
- Mycoplasma Chromosomal Transfer: A Distributive, Conjugative Process Creating an Infinite Variety of Mosaic Genomes.Frontiers in microbiology · 2019Article
- Article
- PGAP-X: extension on pan-genome analysis pipeline.BMC genomics · 2018Article
- Puzzling Over the Pneumococcal Pangenome.Frontiers in microbiology · 2018Review
- Interbacterial predation as a strategy for DNA acquisition in naturally competent bacteria.Nature reviews. Microbiology · 2017Review
- Sequential displacement of Type VI Secretion System effector genes leads to evolution of diverse immunity gene arrays in Vibrio cholerae.Scientific reports · 2017Article
- Not So Simple After All: Bacteria, Their Population Genetics, and Recombination.Cold Spring Harbor perspectives in biology · 2016Review
- Parallel Evolution in Streptococcus pneumoniae Biofilms.Genome biology and evolution · 2016Article
- Comparative Genomic Analysis of Meningitis- and Bacteremia-Causing Pneumococci Identifies a Common Core Genome.Infection and immunity · 2015Article
- Involvement of aph(3')-IIa in the formation of mosaic aminoglycoside resistance genes in natural environments.Frontiers in microbiology · 2015Article
- Heterogeneity in the frequency and characteristics of homologous recombination in pneumococcal evolution.PLoS genetics · 2014Article
- Mechanism of random integration of foreign DNA in transgenic mice.Transgenic research · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Integration of foreign DNA was observed in the Gram-positive human pathogen Streptococcus pneumoniae (pneumococcus) after transformation with DNA from a recombinant Escherichia coli bacteriophage lamda carrying a pneumococcal insert. Segments of lamda DNA replaced chromosomal sequences adjacent to the region homologous with the pneumococcal insert, whence the name insertion-deletion. Here we report that a pneumococcal insert was absolutely required for insertion-deletion formation, but could be as short as 153 bp; that the sizes of foreign DNA insertions (289-2,474 bp) and concomitant chromosomal deletions (45-1,485 bp) were not obviously correlated; that novel joints clustered preferentially within segments of high GC content; and that the crossovers in 29 independent novel joints were located 1 bp from the border or within short (3-10 nt long) stretches of identity (microhomology) between resident and foreign DNA. The data are consistent with a model in which the insert serving as a homologous recombination anchor favors interaction and subsequent illegitimate recombination events at microhomologies between foreign and resident sequences. The potential of homology- directed illegitimate recombination for genome evolution was illustrated by the trapping of functional heterologous genes.
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