ArticleProceedings of the National Academy of Sciences of the United States of America2002
The crystal structure and mutational analysis of a novel RNA-binding domain found in the human Tap nuclear mRNA export factor.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 33 citations in OpenAlex.
- Gammaretroviral pol sequences act in cis to direct polysome loading and NXF1/NXT-dependent protein production by gag-encoded RNA.Retrovirology · 2014Article
- Diffuse transition state structure for the unfolding of a leucine-rich repeat protein.Physical chemistry chemical physics : PCCP · 2014Article
- TREX exposes the RNA-binding domain of Nxf1 to enable mRNA export.Nature communications · 2012Article
- Evolutionary development of redundant nuclear localization signals in the mRNA export factor NXF1.Molecular biology of the cell · 2011Article
- Structure-function studies of nucleocytoplasmic transport of retroviral genomic RNA by mRNA export factor TAP.Nature structural & molecular biology · 2011Article
- Structure-based prediction of RNA-binding domains and RNA-binding sites and application to structural genomics targets.Nucleic acids research · 2011Article
- Formation of a Tap/NXF1 homotypic complex is mediated through the amino-terminal domain of Tap and enhances interaction with nucleoporins.Molecular biology of the cell · 2008Article
- Comparative sequence analysis of leucine-rich repeats (LRRs) within vertebrate toll-like receptors.BMC genomics · 2007Article
- Atomic resolution structures in nuclear transport.Methods (San Diego, Calif.) · 2006Article
- Amino acid residue doublet propensity in the protein-RNA interface and its application to RNA interface prediction.Nucleic acids research · 2006Article
- The mammalian RNA-binding protein Staufen2 links nuclear and cytoplasmic RNA processing pathways in neurons.Neuromolecular medicine · 2004Article
- dsRBM1 and a proline-rich domain of RNA helicase A can form a composite binder to recognize a specific dsDNA.Nucleic acids research · 2003Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The Tap protein mediates the sequence nonspecific nuclear export of cellular mRNAs as well as the sequence-specific export of retroviral mRNAs bearing the constitutive transport element (CTE). Previously, the structures of individual Tap subdomains, including ribonucleoprotein and leucine-rich repeat domains, have been described. Here, we report the crystal structure of a functional CTE RNA-binding domain of human Tap, including the N-terminal arm of the ribonucleoprotein domain and interdomain linking polypeptide. To identify residues that interact with the CTE, we have introduced 38 alanine substitutions for surface residues in the Tap CTE-binding domain and tested these mutants for their ability to support CTE-dependent nuclear RNA export and CTE binding. Four residues that cluster on a concave surface in the leucine-rich repeat domain were found to be critical for CTE binding and define a CTE-interacting surface on this domain. The second critical CTE-interacting surface on Tap is defined by three previously identified residues on the surface of the ribonucleoprotein domain. The structural and mutational data define a novel RNA-binding site on the Tap protein.
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