ArticleProceedings of the National Academy of Sciences of the United States of America2002
A spliceosomal intron in Giardia lamblia.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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70 citing papers in PubMed, 155 citations in OpenAlex.
- Spliceosomal introns in the diplomonad parasiteMicrobial genomics · 2023Article
- Precise gene models using long-read sequencing reveal a unique poly(A) signal inRNA (New York, N.Y.) · 2022Article
- An Unusual U2AF2 Inhibits Splicing and Attenuates the Virulence of the Human Protozoan ParasiteFrontiers in cellular and infection microbiology · 2022Article
- Differential fates of introns in gene expression due to global alternative splicing.Human genetics · 2022Review
- The role of nuclear organization in trans-splicing based expression of heat shock protein 90 in Giardia lamblia.PLoS neglected tropical diseases · 2021Article
- An Intron of Invertebrate Microphthalmia Transcription Factor Gene Is Evolved from a Longer Ancestral Sequence.Evolutionary bioinformatics online · 2021Article
- Eukaryote-Conserved Methylarginine Is Absent in Diplomonads and Functionally Compensated in Giardia.Molecular biology and evolution · 2020Article
- Phylogenetic Analyses of Glycosyl Hydrolase Family 6 Genes in Tunicates: Possible Horizontal Transfer.Genes · 2020Article
- Article
- Patterns of conservation of spliceosomal intron structures and spliceosome divergence in representatives of the diplomonad and parabasalid lineages.BMC evolutionary biology · 2019Article
- Analysis of the Functional Relevance of Epigenetic Chromatin Marks in the First Intron Associated with Specific Gene Expression Patterns.Genome biology and evolution · 2019Article
- Genetic variation in metronidazole metabolism and oxidative stress pathways in clinicalInfection and drug resistance · 2019Article
- Spliceosomal introns in Trichomonas vaginalis revisited.Parasites & vectors · 2018Article
- Sense-antisense gene overlap is probably a cause for retaining the few introns in Giardia genome and the implications.Biology direct · 2018Article
- Characterization and Comparative Analysis of Olfactory Receptor Co-Receptor Orco Orthologs Among Five Mirid Bug Species.Frontiers in physiology · 2018Article
- Multispecies reconstructions uncover widespread conservation, and lineage-specific elaborations in eukaryotic mRNA metabolism.PloS one · 2018Article
- The Macronuclear Genome of Stentor coeruleus Reveals Tiny Introns in a Giant Cell.Current biology : CB · 2017Article
- Interactions between Giardia duodenalis Sm proteins and their association with spliceosomal snRNAs.Parasitology research · 2017Article
- Transcriptomic analysis of diplomonad parasites reveals a trans-spliced intron in a helicase gene inPeerJ · 2017Article
- Review
10 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Short introns occur in numerous protist lineages, but there are no reports of intervening sequences in the protists Giardia lamblia and Trichomonas vaginalis, which may represent the deepest known branches in the eukaryotic line of descent. We have discovered a 35-bp spliceosomal intron in a gene encoding a putative [2Fe-2S] ferredoxin of G. lamblia. The Giardia intron contains a canonical splice site at its 3' end (AG), a noncanonical splice site at its 5' end (CT), and a branch point sequence that fits the yeast consensus sequence of TACTAAC except for the first nucleotide (AACTAAC). We have also identified several G. lamblia genes with spliceosomal peptides, including homologues of eukaryote-specific spliceosomal peptides (Prp8 and Prp11), several DExH-box RNA-helicases that have homologues in eubacteria, but serve essential functions in the splicing of introns in eukaryotes, and 11 predicted archaebacteria-like Sm and like-Sm core peptides, which coat small nuclear RNAs. Phylogenetic analyses show the Giardia Sm core peptides are the products of multiple, ancestral gene duplications followed by divergence, but they retain strong similarity to Sm and like-Sm peptides of other eukaryotes. Although we have documented only a single intron in Giardia, it likely has other introns and fully functional, spliceosomal machinery. If introns were added during eukaryotic evolution (the introns-late hypothesis), then these results push back the date of this event before the branching of G. lamblia.
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