ArticleMolecular biology of the cell2002
Pitx2a expression alters actin-myosin cytoskeleton and migration of HeLa cells through Rho GTPase signaling.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 65 citations in OpenAlex.
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- Paired-like homeodomain 2B contributes to tumour progression and anti-autophagy in human lung cancer.American journal of cancer research · 2021Article
- Identification of Deregulated Pathways, Key Regulators, and Novel miRNA-mRNA Interactions in HPV-Mediated Transformation.Cancers · 2020Article
- TSC1 regulates osteoclast podosome organization and bone resorption through mTORC1 and Rac1/Cdc42.Cell death and differentiation · 2018Article
- Rare and Common Variants Conferring Risk of Tooth Agenesis.Journal of dental research · 2018Article
- Discovery and characterization of small molecule Rac1 inhibitors.Oncotarget · 2017Article
- Current Perspectives in Cardiac Laterality.Journal of cardiovascular development and disease · 2016Review
- Identification of the GTPase-activating protein DEP domain containing 1B (DEPDC1B) as a transcriptional target of Pitx2.Experimental cell research · 2015Article
- Article
- PITX2 and non-canonical Wnt pathway interaction in metastatic prostate cancer.Clinical & experimental metastasis · 2014Article
- Pleiotropic and isoform-specific functions for Pitx2 in superior colliculus and hypothalamic neuronal development.Molecular and cellular neurosciences · 2013Article
- RAC1 GTPase plays an important role in γ-irradiation induced G2/M checkpoint activation.Breast cancer research : BCR · 2012Article
- Article
- Effect of PITX2 knockdown on transcriptome of primary human trabecular meshwork cell cultures.Molecular vision · 2011Article
- Role of a novel coiled-coil domain-containing protein CCDC69 in regulating central spindle assembly.Cell cycle (Georgetown, Tex.) · 2010Article
- A kinome RNAi screen identified AMPK as promoting poxvirus entry through the control of actin dynamics.PLoS pathogens · 2010Article
- Myosin II motor proteins with different functions determine the fate of lamellipodia extension during cell spreading.PloS one · 2010Article
- An altered phenotype in a conditional knockout of Pitx2 in extraocular muscle.Investigative ophthalmology & visual science · 2009Article
- Molecular Signature of HPV-Induced Carcinogenesis: pRb, p53 and Gene Expression Profiling.Current genomics · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We ectopically expressed the transcription factor Pitx2a, one of the Pitx2 isoforms, in HeLa cells by using a tetracycline-inducible expression system and examined whether Pitx2a was capable of modulating Rho GTPase signaling and altering the cell's cytoskeleton. Ectopic expression of Pitx2a induced actin-myosin reorganization, leading to increased cell spreading, suppression of cell migration, and the strengthening of cell-cell adhesion, marked by the accumulation and localization of beta-catenin and N-cadherin to the sites of cell-cell contacts. Moreover, Pitx2a expression resulted in activation of the Rho GTPases Rac1 and RhoA, and the dominant negative Rac1 mutant N17Rac1 inhibited cell spreading and disrupted localization of beta-catenin to the sites of cell-cell contacts. Both reorganization of actin-myosin and cell spreading require phosphatidylinositol 3-kinase activity, which is also necessary for activation of the Rho GTPase proteins. Pitx2a induced the expression of Trio, a guanine nucleotide exchange factor for Rac1 and RhoA, which preceded cell spreading, and the expression of Trio protein was down-regulated after the changes in cell spreading and cell morphology were initiated. In addition, Pitx2a also induces cell cycle arrest at G0/G1, most likely due to the accumulation of the tumor suppressor proteins p53 and p21. Our data indicate that the transcriptional activities initiated in the nucleus by Pitx2a result in profound changes in HeLa cell morphology, migration, and proliferation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.