ArticleMolecular biology of the cell2002
Secretion and assembly of zona pellucida glycoproteins by growing mouse oocytes microinjected with epitope-tagged cDNAs for mZP2 and mZP3.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Furin-Mediated Cleavage of Zona Pellucida Proteins Is Essential for Oocyte Development.MedComm · 2025Article
- Ultrastructure and Transcriptomic Analysis Reveal Alternative Pathways of Zona Radiata Formation inBiology · 2025Article
- Genome-Wide Identification and Expression Analysis of Zona Pellucida (ZP) Gene Family inInternational journal of molecular sciences · 2025Article
- Female fertility and the mammalian egg'sHistology and histopathology · 2024Review
- Article
- Initial detachment of the mouse oocyte from the zona pellucida is mediated by metallopeptidase activity†.Biology of reproduction · 2023Article
- Targeted deletion of liver-expressed Choriogenin L results in the production of soft eggs and infertility in medaka, Oryzias latipes.Zoological letters · 2022Article
- Zona Pellucida Genes and Proteins: Essential Players in Mammalian Oogenesis and Fertility.Genes · 2021Review
- New Insights into the Mammalian Egg Zona Pellucida.International journal of molecular sciences · 2021Review
- Cryo-EM structure of native human uromodulin, a zona pellucida module polymer.The EMBO journal · 2020Article
- Heterozygous mutations in ZP1 and ZP3 cause formation disorder of ZP and female infertility in human.Journal of cellular and molecular medicine · 2020Article
- Mammalian egg coat modifications and the block to polyspermy.Molecular reproduction and development · 2020Review
- Zona pellucida genes and proteins and human fertility.Trends in developmental biology · 2020Article
- Egg Coat Proteins Across Metazoan Evolution.Current topics in developmental biology · 2018Review
- Dosage effects of ZP2 and ZP3 heterozygous mutations cause human infertility.Human genetics · 2017Article
- Sperm proteasomes degrade sperm receptor on the egg zona pellucida during mammalian fertilization.PloS one · 2011Article
- High doses of medroxyprogesterone as the cause of disappearance of adherence of the zona pellucida to an oocyte.Protoplasma · 2010Article
- The pancreatic zymogen granule membrane protein, GP2, binds Escherichia coli Type 1 fimbriae.BMC gastroenterology · 2009Article
- Zona pellucida glycoproteins.The Journal of biological chemistry · 2008Review
- Antibodies to two ZP3 B cell epitopes affect zona pellucida assembly.Journal of reproductive immunology · 2008Article
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3 authors.
Funding
Abstract
The zona pellucida (ZP) is a highly organized extracellular coat that surrounds all mammalian eggs. The mouse egg ZP is composed of three glycoproteins, called mZP1-3, that are synthesized, secreted, and assembled into a ZP exclusively by growing oocytes. Here, we microinjected epitope-tagged (Myc and Flag) cDNAs for mZP2 and mZP3 into the germinal vesicle (nucleus) of growing oocytes isolated from juvenile mice. Specific antibodies and laser scanning confocal microscopy were used to follow nascent, recombinant ZP glycoproteins in both permeabilized and nonpermeabilized oocytes. When such cDNAs were injected, epitope-tagged mZP2 (Myc-mZP2) and mZP3 (Flag-mZP3) were synthesized, packaged into large intracellular vesicles, and secreted by the vast majority of oocytes. Secreted glycoproteins were incorporated into only the innermost layer of the thickening ZP, and the amount of nascent glycoprotein in this region increased with increasing time of oocyte culture. Consistent with prior observations, the putative transmembrane domain at the C terminus of mZP2 and mZP3 was missing from nascent glycoprotein incorporated into the ZP. When the consensus furin cleavage site near the C terminus of mZP3 was mutated, such that it should not be cleaved by furin, secretion and assembly of mZP3 was reduced. On the other hand, mZP3 incorporated into the ZP lacked the transmembrane domain downstream of the mutated furin cleavage site, suggesting that some other protease(s) excised the domain. These results strongly suggest that nascent mZP2 and mZP3 are incorporated into only the innermost layer of the ZP and that excision of the C-terminal region of the glycoproteins is required for assembly into the oocyte ZP.
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