ArticleMolecular biology of the cell2002
Fission yeast Rad26 is a regulatory subunit of the Rad3 checkpoint kinase.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 43 citations in OpenAlex.
- A Novel Role for RNF126 in the Promotion of G2 Arrest via Interaction With 14-3-3σ.International journal of radiation oncology, biology, physics · 2022Article
- A chemical biology strategy to analyze rheostat-like protein kinase-dependent regulation.Chemistry & biology · 2013Article
- Carboxy-terminal phosphorylation sites in Cdc25 contribute to enforcement of the DNA damage and replication checkpoints in fission yeast.Current genetics · 2012Article
- Checkpoint-dependent and -independent roles of Swi3 in replication fork recovery and sister chromatid cohesion in fission yeast.PloS one · 2010Article
- Rad26p, a transcription-coupled repair factor, is recruited to the site of DNA lesion in an elongating RNA polymerase II-dependent manner in vivo.Nucleic acids research · 2010Article
- The fission yeast DNA structure checkpoint protein Rad26ATRIP/LCD1/UVSD accumulates in the cytoplasm following microtubule destabilization.BMC cell biology · 2006Article
- Two-stage mechanism for activation of the DNA replication checkpoint kinase Cds1 in fission yeast.Genes & development · 2006Article
- Dimerization of the ATRIP protein through the coiled-coil motif and its implication to the maintenance of stalled replication forks.Molecular biology of the cell · 2005Article
- Germinating fission yeast spores delay in G1 in response to UV irradiation.BMC cell biology · 2004Article
- Disruption of the Rad9/Rad1/Hus1 (9-1-1) complex leads to checkpoint signaling and replication defects.Oncogene · 2004Article
- ATM and genome maintenance: defining its role in breast cancer susceptibility.Journal of mammary gland biology and neoplasia · 2004Review
- Histone H2A phosphorylation controls Crb2 recruitment at DNA breaks, maintains checkpoint arrest, and influences DNA repair in fission yeast.Molecular and cellular biology · 2004Article
- Quaternary structure of ATR and effects of ATRIP and replication protein A on its DNA binding and kinase activities.Molecular and cellular biology · 2004Article
- Biochemical characterization of DNA damage checkpoint complexes: clamp loader and clamp complexes with specificity for 5' recessed DNA.PLoS biology · 2003Article
- Article
- Fission yeast Rad26 responds to DNA damage independently of Rad3.BMC genetics · 2003Article
- Telomere binding of checkpoint sensor and DNA repair proteins contributes to maintenance of functional fission yeast telomeres.Genetics · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Fission yeast Rad3 is a member of a family of phosphoinositide 3-kinase -related kinases required for the maintenance of genomic stability in all eukaryotic cells. In fission yeast, Rad3 regulates the cell cycle arrest and recovery activities associated with the G2/M checkpoint. We have developed an assay that directly measures Rad3 kinase activity in cells expressing physiological levels of the protein. Using the assay, we demonstrate directly that Rad3 kinase activity is stimulated by checkpoint signals. Of the five other G2/M checkpoint proteins (Hus1, Rad1, Rad9, Rad17, and Rad26), only Rad26 was required for Rad3 kinase activity. Because Rad26 has previously been shown to interact constitutively with Rad3, our results demonstrate that Rad26 is a regulatory subunit, and Rad3 is the catalytic subunit, of the Rad3/Rad26 kinase complex. Analysis of Rad26/Rad3 kinase activation in rad26.T12, a mutant that is proficient for cell cycle arrest, but defective in recovery, suggests that these two responses to checkpoint signals require quantitatively different levels of kinase activity from the Rad3/Rad26 complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.